Dynamic behavior of plant polyphenols in biological membranes

植物多酚在生物膜中的动态行为

• 批准号: 17580115
• 负责人: NAKAYAMA Tsutomu
• 金额: $ 2.24万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580115/
• 关键词: catechin; polyphenol; phospholipid; lipid membrane; liposome; NMR; EGCg; bicelle; リボソーム; 固体MR; EGCG; カテキン類; ECg; 固体NMR; 脂質二十層; バイセル膜

Interaction between tea catechins, such as epicatechin gallate (ECg) and epigallocatechin gallate (EGCg), and isotropic bicelle model lipid membranes was investigated by solution NMR techniques. ^1HNMR measurements provided signals from the B-ring and the galloyl moiety in ECg and EGCg that were obviously shifted, and whose proton T_1 relaxation times were shortened upon interaction of the catechins with the bicelles. These results indicate that the B-ring and the galloyl moiety play an important role in this interaction. Nuclear Overhauser effect spectrometry experiments demonstrated that the B-ring and the galloyl moiety are located near the γ-H in the phospholipid trimethylammonium group. On the basis of these findings, we propose that ECg and EGCg interact with the surface of lipid membranes via the choline moiety.

用溶液核磁共振技术研究了表儿茶素没食子酸酯（ECg）和表没食子儿茶素没食子酸酯（EGCg）与各向同性双分子模型脂膜的相互作用。~ 1HNMR研究表明，儿茶素与胶束相互作用后，ECg和EGCg中的B环和没食子酰基部分的质子T_1弛豫时间明显缩短。这些结果表明，B-环和没食子酰基部分在这种相互作用中起重要作用。核Overhauser效应光谱实验表明，B环和没食子酰基部分位于磷脂三甲铵基团的γ-H附近。在这些研究结果的基础上，我们提出，心电图和EGCg通过胆碱部分与脂质膜的表面相互作用。

项目成果

Advances in Planar Lipid Bilayers and liposomes, Vol. 4

平面脂质双层和脂质体的进展，卷。

• 发表时间: 2006
• 作者: T.Nakayama;K.Kajiya;S.Kumazawa
• 通讯作者: S.Kumazawa

Dynamic behavior of tea catechins interacting with lipid membranes as determined by NMR spectroscopy

• DOI: 10.1021/jf0712402
• 发表时间: 2007-11-28
• 期刊: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
• 影响因子: 6.1
• 作者: Uekusa, Yoshinori;Kamihira, Miya;Nakayama, Tsutomu
• 通讯作者: Nakayama, Tsutomu

Interaction of Plant Polyphenols with Liposomes

• DOI: 10.1016/s1554-4516(06)04004-x
• 发表时间: 2006-01-01
• 期刊: ADVANCES IN PLANAR LIPID BILAYERS AND LIPOSOMES, VOL 4
• 作者: Nakayama, Tsutomu;Kajiya, Katsuko;Kumazawa, Shigenori
• 通讯作者: Kumazawa, Shigenori

Advances in Planar Lipid Bilayers and Liposomes, Vol.4

平面脂质双层和脂质体的进展，第 4 卷

• 发表时间: 2006
• 作者: T.Nakayama;K.Kajiya;S.Kumazawa
• 通讯作者: S.Kumazawa