Ultrasound therapy for soft tissue sarcoma with new quinolone antibiotics as a sonodynamic compound in vivo

使用新型喹诺酮抗生素作为体内声动力化合物对软组织肉瘤进行超声治疗

• 批准号: 17591545
• 负责人: NAGASAWA Hiroyuki
• 金额: $ 1.41万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591545/
• 关键词: Sparfloxacin; Ultrasound; Antitumor effect; Acridine orange

In 2005, we compared an antitumor effect of acridine orange, piroxicam, and 4 kinds of New quinolone (NQ) antibiotics (lomefloxacin hydrochloride, sparfloxacin, ciprofloxacin hydrochloride, gatifloxacin hydrate) in vitro. Survival rate of tumor cells of the mixture of tumor cells and these 6 drugs after the irradiation (2 MHz, 2.0 W, for 30 s) was calculated. Furthermore, to specify the radicals, D-mannitol and L-histidine was used concurrently. NQ antibiotics showed antitumor activities under US irradiation. Especially sparfloxacin (SPFX) showed the best antitumor effect, and concurrent use of L-histidine suppressed the effect. Therefore, we considered that NQ antibiotics would be useful compounds for antitumor activities under US irradiation, and generation of singlet oxygen was involved in the cell damage process. In addition, acridine orange showed a good antitumor effect.In 2006, we investigated a synergistic antitumor effect of US in the presence of SPFX against sarcoma 180 cells in mouse air pouch model. After the injection of tumor cells (1.5 x 10^7) and 0.2 mM solution of SPFX, US (2 MHz, 10 W) was irradiated for 30 s. However, an antitumor effect of US in the presence of SPFX was not apparent neither in histological study nor in survival analysis. Improvement of US apparatus, or a development of better sonodynamic compounds should be required. As a sonodynamic compound, acridine orange might be promising.

2005年，我们比较了吖啶橙、吡罗昔康和4种新型喹诺酮（NQ）抗生素（盐酸洛美沙星、司帕沙星、盐酸环丙沙​​星、水加替沙星）的体外抗肿瘤作用。计算肿瘤细胞与这6种药物的混合物照射（2 MHz，2.0 W，30 s）后肿瘤细胞的存活率。此外，为了指定自由基，同时使用D-甘露醇和L-组氨酸。 NQ 抗生素在美国辐射下显示出抗肿瘤活性。尤其是司帕沙星(SPFX)的抗肿瘤效果最好，与L-组氨酸合用会抑制其效果。因此，我们认为NQ抗生素将是在美国辐射下具有抗肿瘤活性的有用化合物，并且单线态氧的产生参与细胞损伤过程。此外，吖啶橙也表现出良好的抗肿瘤作用。2006年，我们在小鼠气囊模型中研究了US在SPFX存在下对肉瘤180细胞的协同抗肿瘤作用。注射肿瘤细胞（1.5 x 10^7）和0.2 mM SPFX溶液后，US（2 MHz，10 W）照射30 s。然而，在 SPFX 存在下，US 的抗肿瘤作用在组织学研究和生存分析中均不明显。应该需要改进美国设备，或者开发更好的声动力化合物。作为一种声动力化合物，吖啶橙可能很有前途。