Ultrasound therapy for soft tissue sarcoma with new quinolone antibiotics as a sonodynamic compound in vivo

使用新型喹诺酮抗生素作为体内声动力化合物对软组织肉瘤进行超声治疗

• 批准号: 17591545
• 负责人: NAGASAWA Hiroyuki
• 金额: $ 1.41万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591545/
• 关键词: Sparfloxacin; Ultrasound; Antitumor effect; Acridine orange

In 2005, we compared an antitumor effect of acridine orange, piroxicam, and 4 kinds of New quinolone (NQ) antibiotics (lomefloxacin hydrochloride, sparfloxacin, ciprofloxacin hydrochloride, gatifloxacin hydrate) in vitro. Survival rate of tumor cells of the mixture of tumor cells and these 6 drugs after the irradiation (2 MHz, 2.0 W, for 30 s) was calculated. Furthermore, to specify the radicals, D-mannitol and L-histidine was used concurrently. NQ antibiotics showed antitumor activities under US irradiation. Especially sparfloxacin (SPFX) showed the best antitumor effect, and concurrent use of L-histidine suppressed the effect. Therefore, we considered that NQ antibiotics would be useful compounds for antitumor activities under US irradiation, and generation of singlet oxygen was involved in the cell damage process. In addition, acridine orange showed a good antitumor effect.In 2006, we investigated a synergistic antitumor effect of US in the presence of SPFX against sarcoma 180 cells in mouse air pouch model. After the injection of tumor cells (1.5 x 10^7) and 0.2 mM solution of SPFX, US (2 MHz, 10 W) was irradiated for 30 s. However, an antitumor effect of US in the presence of SPFX was not apparent neither in histological study nor in survival analysis. Improvement of US apparatus, or a development of better sonodynamic compounds should be required. As a sonodynamic compound, acridine orange might be promising.

在2005年，我们比较了艾杂丁橙，吡罗酮和4种新型奎诺酮（NQ）抗生素的抗肿瘤作用（Lomefloxacin盐酸盐，尖氟羟霉素，甲状酸羟胺，甲状腺氟化物盐酸，gatifloxacin hydrate）。计算了肿瘤细胞混合物的肿瘤细胞的存活率和辐照后的这6种药物（2 MHz，2.0 W，为30 s）。此外，为指定自由基，同时使用了D-甘露醇和L-齐丁。 NQ抗生素在美国照射下显示抗肿瘤活性。特别是sparfloxacin（SPFX）表现出最佳的抗肿瘤作用，同时使用L-抗原抑制了效果。因此，我们认为NQ抗生素对于美国辐照下的抗肿瘤活性将是有用的化合物，而生成单线氧的产生参与了细胞损伤过程。此外，阿西迪橙色表现出良好的抗肿瘤作用。在2006年，我们研究了在小鼠空气袋模型中SPFX针对SARMA 180细胞的情况下，我们对我们的协同抗肿瘤作用。注射肿瘤细胞（1.5 x 10^7）和SPFX溶液0.2 mM后，将US（2 MHz，10 W）照射30 s。但是，在组织学研究和生存分析中，在SPFX存在下我们的抗肿瘤作用均不明显。应该需要改进美国设备，或者需要开发更好的声动力化合物。作为一种声动力的化合物，Acridine Orange可能会很有希望。