An experimental treatment of soft tissue sarcoma by HIFU (high-intensity focused ultrasound)

HIFU（高强度聚焦超声）治疗软组织肉瘤的实验性治疗

• 批准号: 22591651
• 负责人: NAGASAWA Hiroyuki
• 金额: $ 2.41万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591651/
• 关键词: HIFU; Ultrasound; Sarcoma; Treatment; 収束超音波; 肉腫; 治療; 集束超音波; アポトーシス; リンパ球; 免疫; マクロファージ遊走阻止因子

High-intensity focused ultrasound (HIFU) therapy offers great promise for the treatment of cancer. The histological changes, including the antitumor immunological response, after HIFU treatment was examined in soft tissue sarcoma. Materials and Methods: Sarcoma 180 cells were injected subcutaneously in mice. Approximately 2 weeks after the injection, the tumor was irradiated by a single shot of HIFU. The tumor diameter was measured and the survival rate was observed after treatment. The tumors were resected, and stained with TUNEL stain, tartrate-resistant acid phosphatase (TRAP) stain to detect tumor-associated macrophages, and immunohistochemical stains for CD4 and CD8. Results: The tumor size in the HIFU group was significantly smaller than the control and survival rate was significantly higher. The numbers of TUNEL-, TRAP-, CD4- and CD8-positive cells infiltrating the tumor were significantly higher in the HIFU group. Conclusion: HIFU, even when administered as a single shot, induces apoptosis of tumor cells and intratumoral infiltration of macrophages and lymphocytes.

高强度聚焦超声（HIFU）治疗为癌症的治疗提供了巨大的希望。观察了HIFU治疗后软组织肉瘤的组织学变化，包括抗肿瘤免疫反应。材料与方法：将肉瘤180细胞接种于小鼠皮下。注射后约2周，通过单次HIFU照射肿瘤。治疗后测量肿瘤直径并观察生存率。切除肿瘤，用TUNEL染色，抗酒石酸酸性磷酸酶（TRAP）染色检测肿瘤相关巨噬细胞，免疫组化染色检测CD 4和CD 8。结果：HIFU组肿瘤体积明显小于对照组，生存率明显高于对照组。HIFU组肿瘤浸润的TUNEL-、TRAP-、CD 4-和CD 8-阳性细胞的数量显著较高。结论：即使是单次注射，HIFU也会诱导肿瘤细胞凋亡以及巨噬细胞和淋巴细胞的肿瘤内浸润。

项目成果

〔招待講演〕 永澤博幸:収束超音波による軟部肉腫治療の試み.第2回秋田・札幌合同整形外科セミナー(日本整形外科学会教育研修講演),2012年4月,秋田市

[特邀讲座]长泽博之：使用聚焦超声治疗软组织肉瘤的试验，第2届秋田-札幌联合整形外科研讨会（日本整形外科学会教育培训讲座），2012年4月，秋田市