Investigation of the role of the fibrinolytic system in cardiac remodeling and its clinical relevance

纤溶系统在心脏重构中的作用及其临床意义的研究

• 批准号: 17591623
• 负责人: AKAMATSU Shigeru
• 金额: $ 2.43万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591623/
• 关键词: cardiac myocytes; ischemia; heat shock protein; crstallin; molecular chaperone; 血小板; 血栓; 凝固・線溶系

We investigated the role of the fibrinolytic system in cardiac remodeling and its clinical relevance. αB-crystallin is the most abundant low-molecular-weight heat shock protein in heart and recent studies have demonstrated that it plays a cardioprotective role during myocardial infarction both in vivo and in vitro. On the other hand, platelet-derived growth factor (PDGF), a potent serum mitogen, has been reported to improve cardiac function after myocardial infarction. Using a mouse myocardial infarction model, we investigated whether αB-crystallin is phosphorylated during myocardial infarction and the implication of PDGF-BB. Phosphorylation of αB-crystallin at Ser-59 was time dependently induced and plasma PDGF-BB levels were concomitantly increased. Moreover, PDGF-BB-stimulated phosphorylation of αB-crystallin was suppressed by SB203580, a specific inhibitor of p38 mitogen-activated protein kinase, in primary cultured cardiac myocytes.

我们研究了纤溶系统在心脏重构中的作用及其临床意义。α b -晶体蛋白是心脏中含量最多的低分子热休克蛋白，近年来的研究表明，α b -晶体蛋白在体内和体外均具有心肌梗死时的心脏保护作用。另一方面，血小板衍生生长因子（PDGF），一种有效的血清有丝分裂原，已被报道可改善心肌梗死后的心功能。通过小鼠心肌梗死模型，我们研究了α b -晶体蛋白在心肌梗死过程中是否磷酸化以及PDGF-BB的意义。α b -晶体蛋白Ser-59位点的磷酸化是时间依赖性的，血浆PDGF-BB水平随之升高。此外，在原代培养的心肌细胞中，pdgf - bb刺激的α b -晶体蛋白磷酸化被p38丝裂原活化蛋白激酶特异性抑制剂SB203580抑制。

项目成果

Midazolam suppresses thrombin-induced heat shock protein 27 phosphorylation through inhibition of p38 mitogen-activated protein kinase in cardiac myocytes

• DOI: 10.1002/jcb.20455
• 发表时间: 2005-09-01
• 期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
• 影响因子: 4
• 作者: Tanabe, K;Akamatsu, S;Kozawa, O
• 通讯作者: Kozawa, O

αB-crystallin is phosphorylated during myocardial infarction : involvement of platelet-derived growth factor-BB.

αB-晶状体蛋白在心肌梗塞期间被磷酸化：血小板衍生生长因子-BB 的参与。

• 发表时间: 2005
• 期刊: Arch. Biochem. Biophys. 438
• 作者: Shu E;Matsuno H;Akamatsu S;Kanno Y;Suga H;Nakajima K;Ishisaki A;Kato K;Kitajima Y;Kozawa O
• 通讯作者: Kozawa O

Possible involvement of phosphatidylinositol 3-kinase/Akt signal pathway in vasopressin-induced HSP27 phosphorylation in aortic smooth muscle A10 cells.

磷脂酰肌醇 3-激酶/Akt 信号通路可能参与主动脉平滑肌 A10 细胞中加压素诱导的 HSP27 磷酸化。

• 发表时间: 2005
• 期刊: Arch Biochem Biophys. 438(2)
• 作者: Suga H;Nakajima K;Shu E;Kanno Y;Hirade K;Ishisaki A;Matsuno H;Tanabe K;Takai S;Akamatsu S;Kato K;Oiso Y;Kozawa O.
• 通讯作者: Kozawa O.