The contribution of lung protective ventilation and anti-cytokine therapy in ventilator induced lung injury

肺保护性通气和抗细胞因子治疗在呼吸机所致肺损伤中的作用

• 批准号: 17591628
• 负责人: FUJINO Yuji
• 金额: $ 2.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591628/
• 关键词: Acute lung injury; HFO; mechanical ventilation; proinflammatory cytokine; chemokine receptor; quantitative RT-PCR; 急性呼吸不全; 肺傷害; 高頻度換気法

Prior to the assessment of anti-cytokine therapy, we tried to elucidate the effect of pCO2 on the production of proinflammatory cytokine in order to determine the effect of pCO2 in HFO upon experimental results in animal study.Wistar rats were ventilated and administered HCl intratracheally. After HC1 injection, rats were randomized to two groups: hypercapnia group and normocapnia group. The animals were ventilated with HFO for 4hrs. Lung volume was titrated to achieve PaCO2 40-50 mmHg (normocapnia group) or 80mmHg (hypercapnia group). After 4hrs of mechanical ventilation, the level of TNF-a,IL-6,and CINC-1 in BAL fluid was determined. No significant difference was observed between these two groups. However, W/D ratio and histological study showed that hypercapnia attenuated lung injury.Furthermore, we tried to establish quantitative RT-PCR system of chemokine receptors(CCR4,5,6,7,9,CXCR3,4) by the use of colitis model. Chemokine receptors except CCR6 were upregulated in diseased mice. Interestingly, mRNA of CCR7 and CXCR4 were upregulated in diseased colon compared with non-diseased small intestine.

在评估抗细胞因子治疗之前，我们试图阐明pCO 2对促炎细胞因子产生的影响，以确定HFO中pCO 2对动物研究中实验结果的影响。注射盐酸后，将大鼠随机分为两组：高碳酸血症组和正常碳酸血症组。动物用HFO通气4小时。调整肺容量至PaCO 2 40-50 mmHg（正常碳酸血症组）或80 mmHg（高碳酸血症组）。机械通气4 h后测定BALF中TNF-α、IL-6和CINC-1的水平。两组间无显著性差异。本研究还尝试建立结肠炎模型大鼠趋化因子受体（CCR 4、5、6、7、9、CXCR 3、4）的定量RT-PCR体系。在患病小鼠中，除CCR 6外的趋化因子受体均上调。有趣的是，与未患病的小肠相比，CCR 7和CXCR 4的mRNA在患病的结肠中上调。

项目成果

Vocal cord paralysis after aortic arch surgery with stent-graft placement, a contemporary method of arch surgery

主动脉弓支架置入术后声带麻痹，这是一种现代的弓形手术方法

• 发表时间: 2007
• 期刊: Journal of Vascular Surgery (In press)
• 作者: 成瀬康治;糸満盛憲;内田菜央;Noriyuki Ohta et al.
• 通讯作者: Noriyuki Ohta et al.

Effect of Ono-EI-600 elastase inhibitor on high-tidal-volume-induced lung injury in rats.

• DOI: 10.1007/s00540-005-0369-2
• 发表时间: 2006-01-01
• 期刊: Journal of anesthesia
• 影响因子: 2.8
• 作者: Fujita, Yasuki;Fujino, Yuji;Nishimura, Masaji
• 通讯作者: Nishimura, Masaji

Vocal cord paralysis after aortic arch surgery: Predictors and clinical outcome

• DOI: 10.1016/j.jvs.2005.11.054
• 发表时间: 2006-04-01
• 期刊: JOURNAL OF VASCULAR SURGERY
• 影响因子: 4.3
• 作者: Ohta, N;Kuratani, T;Mori, T
• 通讯作者: Mori, T

High peak inspiratory flow can aggravate ventilator-induced lung injury in rabbits

高峰值吸气流量可加重兔呼吸机所致肺损伤

• 发表时间: 2007
• 期刊: Medical Science Monitoring 15
• 作者: Fujita Y;Fujino Y;Uchiyama A;Mashimo T;Nishimura M
• 通讯作者: Nishimura M

Effect of peak inspiratory flow on gas exchange, pulmonary mechanics, and lung histology in rabbits with injured lungs

吸气峰值流量对肺损伤兔气体交换、肺力学和肺组织学的影响

• 发表时间: 2006
• 期刊: Journal of Anesthesia 20
• 作者: Fujita Y;Maeda Y;Fujino Y;Uchiyama A;Mashimo T;Nishimura M
• 通讯作者: Nishimura M