Gene expression profiles among different surgical stages of endometrial cancer with or without hyperplasia

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Purpose : Type I endometrial cancer is accompanied by hyperplasia and type II endometrial cancer is not. The purpose of our study is to identify genes involved in carcinogenesis of endometrial cancer, especially those differentially expressed by type I and type II cancers among those surgical stages.Experimental Design : Using a cDNA array technique (GeneChip Human Genome U133 Plus 2.0 Array), we examined expression of 38,500 genes in endometrial cancer cells sampled from 31 tumors, and we compared the expression patterns of the tumor cells with or without hyperplasia. Of these, 15 cases were type I cancers and 16 cases were type II cancers. Laser-capture microdissection directed precise separation of cells of interest from stromal cells. In cancer cells, we identified genes differentially expressed by the two types of cancers. Then hierarchical clustering was applied to those identified genes.Results : Of 38,500 genes examined, 2760 genes were identified to have different levels (SD>1) of expression in type I and type II cancers.