Gene expression profiles among different surgical stages of endometrial cancer with or without hyperplasia

伴或不伴增生子宫内膜癌不同手术阶段的基因表达谱

• 批准号: 17591769
• 负责人: SUGIYAMA Yuko
• 金额: $ 1.34万
• 依托单位: Japanese Foundation for cancer research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591769/
• 关键词: cancer; translational research; microarray

Purpose : Type I endometrial cancer is accompanied by hyperplasia and type II endometrial cancer is not. The purpose of our study is to identify genes involved in carcinogenesis of endometrial cancer, especially those differentially expressed by type I and type II cancers among those surgical stages.Experimental Design : Using a cDNA array technique (GeneChip Human Genome U133 Plus 2.0 Array), we examined expression of 38,500 genes in endometrial cancer cells sampled from 31 tumors, and we compared the expression patterns of the tumor cells with or without hyperplasia. Of these, 15 cases were type I cancers and 16 cases were type II cancers. Laser-capture microdissection directed precise separation of cells of interest from stromal cells. In cancer cells, we identified genes differentially expressed by the two types of cancers. Then hierarchical clustering was applied to those identified genes.Results : Of 38,500 genes examined, 2760 genes were identified to have different levels (SD>1) of expression in type I and type II cancers.

目的：I型子宫内膜癌伴有增生，而II型子宫内膜癌则不伴有增生。我们研究的目的是鉴定与子宫内膜癌发生相关的基因，特别是那些手术阶段中I型和II型癌症差异表达的基因。 实验设计：使用cDNA阵列技术（GeneChip Human Genome U133 Plus 2.0 Array），我们检测了从31个肿瘤中取样的子宫内膜癌细胞中38,500个基因的表达，并将肿瘤细胞的表达模式与 或无增生。其中，15例为I型癌症，16例为II型癌症。激光捕获显微切割可将感兴趣的细胞与基质细胞精确分离。在癌细胞中，我们鉴定了两种癌症差异表达的基因。然后对那些已识别的基因应用层次聚类。结果：在检查的 38,500 个基因中，确定了 2760 个基因在 I 型和 II 型癌症中具有不同水平（SD>1）的表达。