Gene expression profiles among different surgical stages of endometrial cancer with or without hyperplasia

目的：I型子宫内膜癌伴增生，II型子宫内膜癌不伴增生.本研究的目的是鉴定与子宫内膜癌发生有关的基因，特别是那些在I型和II型子宫内膜癌手术分期中差异表达的基因。实验设计：使用cDNA阵列技术（基因芯片人类基因组U133加2.0阵列），我们检查了38,500个基因的表达在子宫内膜癌细胞样品从31个肿瘤，并比较有无增生的肿瘤细胞的表达模式。其中，15例为I型癌症，16例为II型癌症。激光捕获显微切割指导从基质细胞中精确分离感兴趣的细胞。在癌细胞中，我们确定了两种癌症差异表达的基因。结果：在检测的38，500个基因中，有2760个基因在I型和II型肿瘤中的表达水平不同（SD>1）。

Purpose : Type I endometrial cancer is accompanied by hyperplasia and type II endometrial cancer is not. The purpose of our study is to identify genes involved in carcinogenesis of endometrial cancer, especially those differentially expressed by type I and type II cancers among those surgical stages.Experimental Design : Using a cDNA array technique (GeneChip Human Genome U133 Plus 2.0 Array), we examined expression of 38,500 genes in endometrial cancer cells sampled from 31 tumors, and we compared the expression patterns of the tumor cells with or without hyperplasia. Of these, 15 cases were type I cancers and 16 cases were type II cancers. Laser-capture microdissection directed precise separation of cells of interest from stromal cells. In cancer cells, we identified genes differentially expressed by the two types of cancers. Then hierarchical clustering was applied to those identified genes.Results : Of 38,500 genes examined, 2760 genes were identified to have different levels (SD>1) of expression in type I and type II cancers.