Exploring molecular pathogenesis related to inflammatory cytokines in protracted otitis media.

探索与迁延性中耳炎炎症细胞因子相关的分子发病机制。

• 批准号: 17591783
• 负责人: WATANABE Takahiro
• 金额: $ 2.11万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591783/
• 关键词: inflammatory cytokine; otitis media; lipopolysaccharide; NF-κB; 滲出性中耳炎; 14員環マクロライド

Bacterial infection in the middle ear causes synthesis of inflammatory cytokines and chemokine. It is believed overproduction of cytokines play a critical role in pathogenesis of protracted otitis media. Bacterial components, such as LPS, accumulated in the middle ear may continue to induce inflammatory response.Human epithelial cell lines were used in the project. Epithelial cells, which are exposed to pathogen components derived from Nontypeable Haemophilus influenzae (NTHi) and LPS, demonstrate activation of NF-κB.The studies using proteasome inhibitor and p38 MAPK inhibitor showed both IKK-IkBα and p38 MAPK pathways are required in NF-κB activation caused by bacterial infection.In conclusion, our studies demonstrate inflammatory response caused by NTHi infection requires both IKK-IκBα and p38 MAPK pathways. Two pathways are also implicated in regulating inflammatory cytokines and chemokine, such as TNF-α, IL-lβ, and IL-8.Our future studies will focus on investigating synthesis of cell adhesion molecules required for the infiltration of inflammatory cells.Our future studies will focus on investigating synthesis of cell adhesion molecules required for the infiltration of inflammatory cells.

中耳细菌感染引起炎性细胞因子和趋化因子的合成。目前认为，细胞因子的过度产生在迁延性中耳炎的发病机制中起关键作用。细菌成分，如脂多糖，积累在中耳可能会继续诱导炎症反应。非分型流感嗜血杆菌（NTHi）和脂多糖（LPS）可激活上皮细胞NF-κB。蛋白酶体抑制剂和p38 MAPK抑制剂的研究表明，IKK-Ik B α和p38 MAPK通路均参与了细菌感染引起的NF-κB激活。我们的研究表明，NTHi感染引起的炎症反应需要IKK-IκBα和p38 MAPK途径。另外，两条途径也参与了炎症细胞因子和趋化因子的调节，如TNF-α、IL-1 β和IL-8。我们未来的研究将集中于炎症细胞浸润所需的细胞粘附分子的合成，我们未来的研究将集中于炎症细胞浸润所需的细胞粘附分子的合成。

项目成果

アンケート調査による網膜色素変性症の難聴・耳鳴の頻度

基于问卷调查的色素性视网膜炎患者听力损失和耳鸣发生频率

• 发表时间: 2006
• 期刊: Otol Jpn 16(1)
• 作者: 岩崎 聡;他
• 通讯作者: 他

Prevalence of hearing loss in retinitis pigmentosa

视网膜色素变性听力损失的患病率

• 发表时间: 2006
• 期刊: Otol Jpn 16(1)
• 作者: Satoshi Iwasaki;et. al.
• 通讯作者: et. al.

Tick bites in the external auditory canal

• DOI: 10.1016/j.anl.2006.09.013
• 发表时间: 2007-09-01
• 期刊: AURIS NASUS LARYNX
• 影响因子: 1.7
• 作者: Iwasaki, Satoshi;Takebayashi, Satoru;Watanabe, Takahiro
• 通讯作者: Watanabe, Takahiro

鼓室型グロームス腫瘍の2症例術前血管塞栓術に関する考察

鼓室血管瘤术前血管栓塞治疗二例探讨

• 发表时间: 2007
• 期刊: 鼓室型グロームス臨の2症例術前血管塞栓術に関する考察 17(5)
• 作者: 渡邉 高弘;他
• 通讯作者: 他

Two Cases of Glomus Tympanicum Tumor : Determining the Need for Preoperative Embolization in Patients with Glomus Tympanicum Tumor

鼓室肿瘤两例：确定鼓室肿瘤患者术前栓塞的必要性

• 发表时间: 2007
• 期刊: Otol Jpn 17(5)
• 作者: Watanabe T;et. al.
• 通讯作者: et. al.