Roles of co-stimulatory molecules in the development of murine allergic conjunctivitis

共刺激分子在小鼠过敏性结膜炎发生中的作用

基本信息

  • 批准号:
    17591838
  • 负责人:
  • 金额:
    $ 1.73万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2006
  • 项目状态:
    已结题

项目摘要

We have demonstrated that Ag-specific T cells play a predominant role in the development of experimental allergic conjunctivitis (EC) in mice. Activation of T cells requires the signal from co-stimulatory molecules. Therefore, we aimed to investigate the roles of co-stimulatory molecules related to T-cell activation play in the development of EC. EC was induced in BALB/c mice by active immunization with ragweed (RW) or passive immunization by transfer of RW-primed splenocytes followed by RW challenge in eye drops. Twenty-four hours after the RW challenge, the conjunctivas were harvested for histological analysis to determine the conjunctival infiltrating eosinophil numbers. To investigate the involvement of co-stimulatory molecules, antibodies (Abs) against co-stimulatory molecules were injected into EC-developing mice either during the induction or the effector phase. During the induction phase, treatment with anti-4-1BB Ab or anti-OX40L Ab suppressed EC, while anti-OX40 Ab treatment augmented EC. During the effector phase, treatment with anti-4-lBB Ab suppressed EC, whereas treatment with anti-B7DC Ab augmented EC. On the contrary, treatment with anti-B7RP-l Ab did not significantly affect EC. These results demonstrate that each co-stimulatory molecule differently participates in the development of EC. With regard to VLA-4 and VCAM-1 which play an important role in eosinophil infiltration, VLA-4 positive cells infiltrated into the conjunctiva by the induction of EC. Blocking of both VLA-4 and VCAM-1 inhibited conjunctival eosinophil infiltration. Thus, the interaction between VLA-4 and VCAM-1 plays a critical role in the development of EC.
我们已经证明,Ag特异性T细胞在小鼠实验性过敏性结膜炎(EC)的发展中起着主导作用。T细胞的激活需要来自共刺激分子的信号。因此,我们的目的是研究与T细胞活化相关的共刺激分子在EC发生中的作用。用豚草(RW)主动免疫BALB/c小鼠,或通过转移RW引发的脾细胞,然后用RW滴眼液攻击的被动免疫,诱导EC。RW激发后24小时,收获结膜进行组织学分析,以确定结膜浸润嗜酸性粒细胞数量。为了研究共刺激分子的参与,在诱导期或效应期将针对共刺激分子的抗体(Abs)注射到EC发育小鼠中。在诱导期,抗4-1BB抗体或抗OX 40 L抗体治疗抑制EC,而抗OX 40抗体治疗增强EC。在效应期,用抗4- 1BB Ab治疗抑制EC,而用抗B7 DC Ab治疗增强EC。相反,用抗B7 RP-1 Ab处理不显著影响EC。这些结果表明,每个共刺激分子不同地参与EC的发展。VLA-4和VCAM-1在嗜酸性粒细胞浸润中起重要作用,EC诱导VLA-4阳性细胞浸润到结膜。阻断VLA-4和VCAM-1均抑制结膜嗜酸性粒细胞浸润。因此,VLA-4和VCAM-1之间的相互作用在EC的发展中起着关键作用。

项目成果

期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Role of VLA-4 in the development of allergic conjunctivitis in mice.
  • DOI:
  • 发表时间:
    2006-04
  • 期刊:
  • 影响因子:
    2.2
  • 作者:
    A. Fukushima;Tomoko Yamaguchi;W. Ishida;K. Fukata;H. Ueno
  • 通讯作者:
    A. Fukushima;Tomoko Yamaguchi;W. Ishida;K. Fukata;H. Ueno
Engagement of 4-1BB inhibits the development of experimental allergic conjunctivitis in mice
  • DOI:
    10.4049/jimmunol.175.8.4897
  • 发表时间:
    2005-10-15
  • 期刊:
  • 影响因子:
    4.4
  • 作者:
    Fukushima, A;Yamaguchi, T;Ueno, H
  • 通讯作者:
    Ueno, H
眼表面の免疫,眼科プラクティス 6眼科臨床に必要な解剖整理
眼表免疫,眼科实践 6 眼科临床实践所需的解剖学
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sagara N;Kawaji T;Takano A;Inomata Y;Inatani M;Fukushima M;Tanihara H.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Naoki Kumagai et al.;Fukushima A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Ying Lu et al.;Ken Fukuda et al.;Fukushima A et al.;Ken Fukuda et al.;Ying Lu et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Naoki Kumagai et al.;Ozaki A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Fukushima A et al.;Ying Lu et al.;福島 敦樹
  • 通讯作者:
    福島 敦樹
The interaction between ICOS and B7RP-1 is not required for the development of experimental murine allergic conjunctivitis.
ICOS 和 B7RP-1 之间的相互作用对于实验性小鼠过敏性结膜炎的发展不是必需的。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sagara N;Kawaji T;Takano A;Inomata Y;Inatani M;Fukushima M;Tanihara H.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Naoki Kumagai et al.;Fukushima A et al.
  • 通讯作者:
    Fukushima A et al.
Involvement of programmed death-ligand 2 (PD-L2) in the development of experimental allergic conjunctivitis in mice
  • DOI:
    10.1136/bjo.2006.091314
  • 发表时间:
    2006-08-01
  • 期刊:
  • 影响因子:
    4.1
  • 作者:
    Fukushima, A.;Yamaguchi, T.;Ueno, H.
  • 通讯作者:
    Ueno, H.
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FUKUSHIMA Atsuki其他文献

FUKUSHIMA Atsuki的其他文献

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{{ truncateString('FUKUSHIMA Atsuki', 18)}}的其他基金

Therapeutic approach against allergic conjunctivitis by oral immune tolerance
口服免疫耐受治疗过敏性结膜炎的方法
  • 批准号:
    16K11324
  • 财政年份:
    2016
  • 资助金额:
    $ 1.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of the role of regulatory T cells in autoimmune ocular inflammatory diseases
阐明调节性 T 细胞在自身免疫性眼部炎症性疾病中的作用
  • 批准号:
    25462757
  • 财政年份:
    2013
  • 资助金额:
    $ 1.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Roles of macrophages in the development of allergic conjunctival diseases
巨噬细胞在过敏性结膜疾病发展中的作用
  • 批准号:
    21592259
  • 财政年份:
    2009
  • 资助金额:
    $ 1.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of a role antigen-presenting cells play in the development of allergic conjunctival diseases
分析抗原呈递细胞在过敏性结膜疾病发生中的作用
  • 批准号:
    19592025
  • 财政年份:
    2007
  • 资助金额:
    $ 1.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Measurement of impedance in vivo in eye lids-development of examination of malignant lid tumors
眼睑体内阻抗测量-眼睑恶性肿瘤检查的进展
  • 批准号:
    14571675
  • 财政年份:
    2002
  • 资助金额:
    $ 1.73万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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