The role of transcriptional factor in neovascularization of proliferative diabetic retinopathy

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To assess the potential role of transcriptional factor in nevascularization of proliferative diabetic retinopathy (PDR), we examined the expression of activator protein-1 (AP-1) and nuclear factor kappa B (NFkB) in epiretinal membrane (ERM) of PDR and performed in vitro studies.Expression of AP-1 and NFkB in PDR ERMs1) AP-1 mRNA was detected in all (100%) of the 19 eyes with PDR, but in only 7 (44%) of the 16 eyes with idiopathic ERM (P<0.05). NFkB mRNA was detected in 14 (74%) of the 19 eyes with PDR, but in only 6 (38%) of the 16 eyes with idiopathic ERM (P<0.05).2) AP-1 protein was detected in PDR ERMs and was coexpressed with the glial-specific marker vimentin. Also, NFkB protein was detected in PDR ERMs and was coexpressed with vimentin and MCP-1.in vitro study1) Glycated albumin stimulation significantly increased AP-1 phosphorylation in glial cells. Also, glycated albumin stimulation induced NFkB activationation and translocation to nuclei in glial cells.