The role of transcriptional factor in neovascularization of proliferative diabetic retinopathy

转录因子在增殖性糖尿病视网膜病变新生血管形成中的作用

• 批准号: 17591846
• 负责人: MITAMURA Yoshinori
• 金额: $ 2.18万
• 依托单位: Chiba University (2006)Sapporo Medical University (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591846/
• 关键词: Diabetic retinopathy; Neovascularization; Transcriptional factor

To assess the potential role of transcriptional factor in nevascularization of proliferative diabetic retinopathy (PDR), we examined the expression of activator protein-1 (AP-1) and nuclear factor kappa B (NFkB) in epiretinal membrane (ERM) of PDR and performed in vitro studies.Expression of AP-1 and NFkB in PDR ERMs1) AP-1 mRNA was detected in all (100%) of the 19 eyes with PDR, but in only 7 (44%) of the 16 eyes with idiopathic ERM (P<0.05). NFkB mRNA was detected in 14 (74%) of the 19 eyes with PDR, but in only 6 (38%) of the 16 eyes with idiopathic ERM (P<0.05).2) AP-1 protein was detected in PDR ERMs and was coexpressed with the glial-specific marker vimentin. Also, NFkB protein was detected in PDR ERMs and was coexpressed with vimentin and MCP-1.in vitro study1) Glycated albumin stimulation significantly increased AP-1 phosphorylation in glial cells. Also, glycated albumin stimulation induced NFkB activationation and translocation to nuclei in glial cells.

为了评估转录因子在增殖性糖尿病视网膜病变 (PDR) 血管形成中的潜在作用，我们检测了 PDR 视网膜前膜 (ERM) 中激活蛋白 1 (AP-1) 和核因子 kappa B (NFkB) 的表达，并进行了体外研究。PDR ERM 中 AP-1 和 NFkB 的表达1) 在所有 (100%) 视网膜前膜 (ERM) 中均检测到 AP-1 mRNA。 19 只眼患有 PDR，但 16 只眼中只有 7 只（44%）患有特发性 ERM（P<0.05）。 19 只眼中 PDR 的 14 只眼（74%）检测到 NFkB mRNA，但 16 只眼特发性 ERM 中仅 6 只眼（38%）检测到 NFkB mRNA（P<0.05）。2）在 PDR ERM 中检测到 AP-1 蛋白，并与胶质细胞特异性标志物波形蛋白共表达。此外，在 PDR ERM 中检测到 NFkB 蛋白，并与波形蛋白和 MCP-1 共表达。体外研究1) 糖化白蛋白刺激显着增加神经胶质细胞中的 AP-1 磷酸化。此外，糖化白蛋白刺激诱导 NFkB 激活并易位至神经胶质细胞的细胞核。

项目成果

The role of cytokine and trophic factors in epiretinal membranes : involvement of signal transduction in glial cells.

细胞因子和营养因子在视网膜前膜中的作用：神经胶质细胞信号转导的参与。

• 发表时间: 2006
• 期刊: Prog Retin Eye Research 25
• 作者: Harada C;Okumura A;Namekata K;Nakamura K;Mitamura Y;Ohguro H;Harada T;Mitamura Y;Harada C
• 通讯作者: Harada C

Role of monocyte chemotactic protein-1 and nuclear factor kappa B in the pathogenesis of proliferative diabetic retinopathy

• DOI: 10.1016/j.diabres.2006.04.017
• 发表时间: 2006-12-01
• 期刊: DIABETES RESEARCH AND CLINICAL PRACTICE
• 影响因子: 5.1
• 作者: Harada, Chikako;Okumura, Akinori;Harada, Takayuki
• 通讯作者: Harada, Takayuki

Activator protein-1 in epiretinal membrane of patients with proliferative diabetic retinopathy.

增殖性糖尿病视网膜病变患者视网膜前膜中的激活蛋白-1。

• 发表时间: 2006
• 期刊: Diabetologia 49
• 作者: Harada C;Okumura A;Namekata K;Nakamura K;Mitamura Y;Ohguro H;Harada T;Mitamura Y
• 通讯作者: Mitamura Y

Activator protein-1 in epiretinal membranes of patients with proliferative diabetic retinopathy

• DOI: 10.1007/s00125-005-0059-5
• 发表时间: 2005
• 期刊: Diabetologia
• 影响因子: 8.2
• 作者: Y. Mitamura;A. Okumura;C. Harada;K. Namekata;K. Nakamura;A. Tashimo;K. Ohtsuka;T. Harada