权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on synthesizing of intracellular polysaccharide by Streptococcus mutans in dentinal tubules.

牙本质小管内变形链球菌合成胞内多糖的研究

基本信息

批准号：
17591993
负责人：
OZAKI Kazumi
金额：
$ 2.24万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591993/
关键词：
Dentinal caries Streptococcus mutans Intracellular polysaccharide Quorum sensing system

项目摘要

Streptococcus mutans that is one of the cariogenic bacterium is synthesized the water-soluble glucan (WSG) and the water-insoluble glucan (WIG) from sucrose, adheres on the tooth, and proliferates. In this study, S. mutans wild-type strain UA159 and its knockout mutants, which were defective in comC, comX and scrA mutant, were examined for their ability for initial adhesion, glucan synthesis and invasion into dentinal tubles. The proportions of water-insoluble glucan synthesis in all mutant strains were less than the wild type. The proportions of water-soluble glucan adhering to dentin slices in all mutant strains were also less than in the wild type. The total dose of three kinds of glucan composition decreased in all mutant strains. The expansion rates of dentinal tubules by comC and comX mutant increased more than by the wild type when cultured in brain heart infusion for 24 days. There were no detectable difference in growth between wild-type and scrA-mutant. In the presence of sucrose, the produce of WSG and WIG in scrA-mutant was less than in wild-type. This study demonstrated that the quorum-sensing system and PTS system may play a crucial role in glucan synthesis, biofilm formation, production of intracellular-polysaccharide and invasion to dentinal tubules of S.mutans.

作为致龋菌之一的变形链球菌由蔗糖合成水溶性葡聚糖(WSG)和水不溶性葡聚糖(WIG)，附着在牙齿上并增殖。在本研究中，检测了变形链球菌野生型菌株UA159及其敲除突变体（其具有comC、comX和scrA突变体缺陷）的初始粘附、葡聚糖合成和侵入牙本质小管的能力。所有突变株中水不溶性葡聚糖合成的比例均低于野生型。所有突变株中粘附在牙本质切片上的水溶性葡聚糖的比例也低于野生型。所有突变株中三种葡聚糖成分的总剂量均下降。当在脑心浸液中培养24天时，comC和comX突变体的牙本质小管的扩张率比野生型增加更多。野生型和scrA突变体之间的生长没有可检测到的差异。在蔗糖存在下，scrA突变体中WSG和WIG的产生量低于野生型。本研究表明，群体感应系统和PTS系统可能在变形链球菌的葡聚糖合成、生物膜形成、细胞内多糖的产生以及对牙本质小管的侵袭中发挥关键作用。

项目成果

期刊论文数量（30）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Cytokines differentially regulate ICAM-1 and VCAM-1 expression on human gingival fibroblasts

DOI：
10.1111/j.1365-2249.2006.03064.x
发表时间：
2006-06-01
期刊：
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
影响因子：
4.6
作者：
Hosokawa, Y;Hosokawa, I;Matsuo, T
通讯作者：
Matsuo, T

Langerhans細胞における抗菌ペプチドの発現について

关于抗菌肽在朗格汉斯细胞中的表达

DOI：
发表时间：
2005
期刊：
日本歯周病学会会誌 47秋季特別号
影响因子：
0
作者：
Nakaoki Y;Sasakawa W;Noda M;Inoue S;Komatsu N;Sano H.;Unemori M et al.;Unemori M et al.;Uemori M et al.;Hosokawa Y. et al.;Hosokawa Y. et al.;細川義隆ら;細川義隆ら;藤坂菊美ら;Hosokawa Y.et al.;Hosokawa Y. et al.;Hosokawa Y.et al.;Hosokawa Y.et al.;Fujisaka K.et al.;Hosokawa Y. et al.;Hosokawa Y. et al.;細川義孝ら;細川義孝ら;藤坂菊美ら;細川育子ら
通讯作者：
細川育子ら

Proinflammatory effects of tumour necrosis factor-like weak inducer of apoptosis (TWEAK) on human gingival fibroblasts

DOI：
10.1111/j.1365-2249.2006.03233.x
发表时间：
2006-12-01
期刊：
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
影响因子：
4.6
作者：
Hosokawa, Y.;Hosokawa, I.;Matsuo, T.
通讯作者：
Matsuo, T.

Cytokines differentially regulate ICAM-land VCAM-1expression on human gingival fibroblasts

细胞因子差异调节人牙龈成纤维细胞上的 ICAM 和 VCAM-1 表达

DOI：
发表时间：
2006
期刊：
Clinical and Experimental Immunology 144・3
影响因子：
0
作者：
Nakaoki Y;Sasakawa W;Noda M;Inoue S;Komatsu N;Sano H.;Unemori M et al.;Unemori M et al.;Uemori M et al.;Hosokawa Y. et al.
通讯作者：
Hosokawa Y. et al.

Proinflammatory effects of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) on human gingival fibroblasts

肿瘤坏死因子样弱凋亡诱导剂（TWEAK）对人牙龈成纤维细胞的促炎作用

DOI：
发表时间：
2006
期刊：
Clinical and Experimental Immunology Vol.146, No.3
影响因子：
0
作者：
Nakaoki Y;Sasakawa W;Noda M;Inoue S;Komatsu N;Sano H.;Unemori M et al.;Unemori M et al.;Uemori M et al.;Hosokawa Y. et al.;Hosokawa Y. et al.;細川義隆ら;細川義隆ら;藤坂菊美ら;Hosokawa Y.et al.;Hosokawa Y. et al.
通讯作者：
Hosokawa Y. et al.