Dynamics, kinetics and assembly of model intrinsically disordered proteins from a polymer physics perspective

从聚合物物理学的角度研究模型本质无序蛋白质的动力学、动力学和组装

• 批准号: 490662871
• 负责人: Professor Dr. Frank Schreiber
• 金额: --
• 依托单位: Institut für Angewandte Physik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/490662871?language=en
• 关键词: Dynamics; kinetics; assembly; model; intrinsically

Supramolecular assemblies of intrinsically disordered proteins (IDPs), i.e. of proteins lacking a stable 3D-structure, are important in cellular signaling and spatial organization and can be involved in pathologies. Here, we wish to explore the dynamics and kinetics of such assembly processes of IDPs to understand the fundamental mechanisms behind IDP-related diseases. Using the model systems α, ß, and kappa-casein (all disordered), ß-lactoglobulin and apo-myoglobin (both ordered), we intend to establish their respective phase diagrams in solution, to determine the parameter space leading to the formation of supramolecular IDP assemblies, and to quantify the influence of protein disorder on these assembly pathways, as well as to compare chemically and thermally denatured proteins. This projectsystematically combines advanced x-ray and neutron scattering methods, notably accessing dynamics on the molecular level during kinetics, as well as theory.

内在无序蛋白（IDP）的超分子组装，即缺乏稳定的3D结构的蛋白质，在细胞信号传导和空间组织中是重要的，并且可以参与病理学。在这里，我们希望探索这种组装过程的动态和动力学的国内流离失所者，以了解国内流离失所者相关疾病背后的基本机制。使用模型系统α，β，κ-酪蛋白（全部无序），β-乳球蛋白和脱辅基肌红蛋白（均有序），我们打算建立各自的相图在溶液中，以确定参数空间导致形成的超分子IDP组装，并量化这些组装途径上的蛋白质无序的影响，以及比较化学和热变性的蛋白质。该项目系统地结合了先进的X射线和中子散射方法，特别是在动力学和理论过程中访问分子水平上的动力学。