Dynamics, kinetics and assembly of model intrinsically disordered proteins from a polymer physics perspective

内在无序蛋白质(IDPs)的超分子组装，即缺乏稳定的三维结构的蛋白质，在细胞信号和空间组织中具有重要作用，并可参与病理过程。在这里，我们希望探索国内流离失所者这种组装过程的动力学和动力学，以了解与国内流离失所者相关的疾病背后的基本机制。利用模型体系α、？和Kappa-Casein(都是有序的)、？-乳球蛋白和apo-肌红蛋白(都是有序的)，我们打算建立它们各自在溶液中的相图，确定导致超分子IdP组装形成的参数空间，并量化蛋白质无序对这些组装途径的影响，以及比较化学和热变性蛋白质。该项目系统地结合了先进的X射线和中子散射方法，特别是在动力学过程中获得分子水平上的动力学，以及理论。

Supramolecular assemblies of intrinsically disordered proteins (IDPs), i.e. of proteins lacking a stable 3D-structure, are important in cellular signaling and spatial organization and can be involved in pathologies. Here, we wish to explore the dynamics and kinetics of such assembly processes of IDPs to understand the fundamental mechanisms behind IDP-related diseases. Using the model systems α, ß, and kappa-casein (all disordered), ß-lactoglobulin and apo-myoglobin (both ordered), we intend to establish their respective phase diagrams in solution, to determine the parameter space leading to the formation of supramolecular IDP assemblies, and to quantify the influence of protein disorder on these assembly pathways, as well as to compare chemically and thermally denatured proteins. This projectsystematically combines advanced x-ray and neutron scattering methods, notably accessing dynamics on the molecular level during kinetics, as well as theory.