Two-Components Colloidal Solutions: Tuning the Interactions of Functionalized Nanoparticles with Proteins

双组分胶体溶液：调节功能化纳米粒子与蛋白质的相互作用

• 批准号: 123578767
• 负责人: Professor Dr. Frank Schreiber
• 金额: --
• 依托单位: Lehrstuhl Physik der molekularen und biologischen Materie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/123578767?language=en
• 关键词: Two; Components; Colloidal; Solutions; Tuning

Nanoparticles (NPs) functionalized by self-assembled monolayers (SAMs) and proteins (known as bioconjugates) are widely used in many bio-related areas, such as biomolecular detection and diagnostic applications. From the point of view of colloidal physics, understanding the phase behavior of these two-component (NPs plus proteins) systems is a challenge, especially given the non-trivial pair-wise interactions and their different response to environmental parameters. In this proposal, we will address the questions: What is the conformation/structure of specific SAM-forming molecules on NPs, and of adsorbed proteins at SAM-solvent interfaces? How do these affect the interactions and phase behavior of colloidprotein solutions? How do proteins interact with themselves as well as with functionalized NPs in solution? What are the key parameters determining the phase behavior of this two-component system (nature and concentration of salt, pH, T, …)? In order to study the interactions and phase behavior of protein-colloid mixtures in general, several typical proteins, specifically albumin, enzymes and antibodies will be used as those proteins are used exhaustively in biosensors. A set of complementary experimental techniques including small-angle scattering (SAXS, SANS), dynamic light scattering (DLS), and spectroscopy (FTIR, UV-vis and circular dichroism) will be used to address the different issues in these complex systems.

由自组装单层 (SAM) 和蛋白质（称为生物共轭物）功能化的纳米颗粒 (NP) 广泛应用于许多生物相关领域，例如生物分子检测和诊断应用。从胶体物理学的角度来看，理解这些双组分（纳米粒子加蛋白质）系统的相行为是一个挑战，特别是考虑到非平凡的成对相互作用及其对环境参数的不同响应。在本提案中，我们将解决以下问题：NP 上特定 SAM 形成分子以及 SAM 溶剂界面处吸附蛋白质的构象/结构是什么？这些如何影响胶体蛋白溶液的相互作用和相行为？蛋白质如何与自身以及溶液中的功能化纳米粒子相互作用？决定这个双组分系统相行为的关键参数是什么（盐的性质和浓度、pH、T……）？为了研究蛋白质-胶体混合物的相互作用和相行为，将使用几种典型的蛋白质，特别是白蛋白、酶和抗体，因为这些蛋白质在生物传感器中被广泛使用。一系列互补的实验技术，包括小角散射（SAXS、SANS）、动态光散射（DLS）和光谱（FTIR、紫外可见光和圆二色性）将用于解决这些复杂系统中的不同问题。

项目成果

Gold nanoparticles decorated with oligo(ethylene glycol) thiols: surface charges and interactions with proteins in solution.

• DOI: 10.1016/j.jcis.2014.03.052
• 发表时间: 2014-07
• 期刊: Journal of colloid and interface science
• 影响因子: 9.9
• 作者: Moritz Schollbach;Fajun Zhang;F. Roosen‐Runge;M. Skoda;R. Jacobs;F. Schreiber