Protein Adsorption Tuned by Multivalent Ions (PATMI):Connecting Bulk to Interface Behaviour by Controlling Interactions

多价离子调节的蛋白质吸附 (PATMI)：通过控制相互作用将体相与界面行为联系起来

• 批准号: 431858909
• 负责人: Professor Dr. Frank Schreiber
• 金额: --
• 依托单位: Lehrstuhl Physik der molekularen und biologischen Materie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/431858909?language=en
• 关键词: Protein; Adsorption; Tuned; Multivalent; Ions

This proposal aims to employ the rich bulk phase behaviour of protein solutions including re-entrant condensation mediated by multivalent ions to elucidate the fundamentals of protein adsorption at interfaces beyond simple charge screening.This qualitatively new behaviour will be explored both at equilibrium (i.e. adsorption isotherms will be determined along with the equilibrium structures) and dynamically (and therefore prior to equilibrium, i.e. the adsorption kinetics will be investigated), with particular attention to clarify the role of surface-induced nucleation and its importance for protein crystallisation.We aim to connect the interface behaviour to the interactions controlled by multivalent ions and the bulk behaviour within the concept of ion-activated patches.We make use of our expertise in surface analysis techniques, such as ellipsometry, QCM-D, FTIRRAS/ATR and in particular neutron and X-ray scattering, which allow a detailed determination of the adsorbed layer structure at the molecular level.

该提案旨在采用蛋白质溶液的丰富体积相位行为，包括由多价离子介导的重进入凝结，以阐明在超出简单充电筛选的范围内蛋白质吸附的基本原理。在质量上，既定的新行为均应在平衡处探索与均衡的均衡（即，在均衡的结构中都可以在均衡中确定均等（即，均一度均具有均匀的启发性），从而在均等均设有动动脉群，因此，一式动脉均取得了启动（即），因此，均一度的均匀效果（即），因此，在均衡（即），均应依次（即）均匀（即），均应在均衡状态。 adsorption kinetics will be investigated), with particular attention to clarify the role of surface-induced nucleation and its importance for protein crystallisation.We aim to connect the interface behaviour to the interactions controlled by multivalent ions and the bulk behaviour within the concept of ion-activated patches.We make use of our expertise in surface analysis techniques, such as ellipsometry, QCM-D, FTIRRAS/ATR and in particular中子和X射线散射，可以详细测定分子水平的吸附层结构。