Structural and morphological characterization of ceramide-1-phosphate model membranes

1-磷酸神经酰胺模型膜的结构和形态表征

• 批准号: 49496884
• 负责人: Professor Dr. Gerald Brezesinski
• 金额: --
• 依托单位: Abteilung Kolloidchemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/49496884?language=en
• 关键词: Structural; morphological; characterization; ceramide; phosphate

Ceramide-1-phosphate has been identified as an important signaling molecule that mediates a large variety of important physiological functions, but its physicochemical properties and how these properties are linked to its diverse physiological functions have not been detailed. The influence of a variety of environmental factors like salt concentration, presence of divalent cations and presence of other lipids will be investigated by using broad range of modern and sophisticated structural, thermodynamic and morphological methods. The proposed research strongly benefits from the complementary expertise of the two research groups. Ceramide-1-phosphate mediated signaling events have been associated in many cases with cholesterol/sphingolipid enriched domains (“rafts”). While interaction with cationic species is expected to result in the formation of higher order domains within the liquid-ordered lipid raft domain, it is likely that these ordered structures disintegrate once repulsive forces become dominant. The formation of highly ordered domains would most likely restrict the interaction with proteins, i.e., the headgroup would function like an electrostatic switch. It is suggested that ceramide-1-phosphate and phosphoinositides are co-localized under certain circumstances. Therefore, the proposed research aims to explore the interaction between these important signaling molecules.

神经酰胺-1-磷酸已被鉴定为介导多种重要生理功能的重要信号分子，但其理化性质以及这些性质如何与其多种生理功能相关联尚未详细说明。各种环境因素的影响，如盐浓度，二价阳离子的存在和其他脂质的存在下，将通过使用广泛的现代和复杂的结构，热力学和形态学方法进行研究。拟议的研究大大受益于两个研究小组的互补专业知识。在许多情况下，神经酰胺-1-磷酸介导的信号传导事件与胆固醇/鞘脂富集结构域（“筏”）相关。虽然预期与阳离子物质的相互作用会导致在液体有序的脂筏结构域内形成更高级的结构域，但一旦排斥力占主导地位，这些有序结构就可能解体。高度有序结构域的形成很可能会限制与蛋白质的相互作用，即，头组的功能就像静电开关一样。这表明，神经酰胺-1-磷酸和磷酸肌醇是共同定位在某些情况下。因此，拟议的研究旨在探索这些重要信号分子之间的相互作用。