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Transposition and gene regulation of DNA transposable elements in plants

植物DNA转座元件的转座和基因调控

基本信息

批准号：
10216208
负责人：
IIDA Shigeru
金额：
$ 29.57万
依托单位：
Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2002
项目状态：
已结题

项目摘要

The major DNA-based controlling transposable elements in higher plants can be classified into three groups : the Ac/Ds (or hAT) superfamily, the En/Spm (or CACTA) superfamily, and the Mu family. As floricultural plants, spontaneous mutants of the common morning glory (I. purpurea) displaying either white or reddish flowers were obtained while various spontaneous mutants of the Japanese morning glory (I. nil) exhibiting many different flower colorations were generated. We are trying to identify these spontaneous mutants including mutable alleles conferring variegated flowers. In addition, we also attempted gene targeting by homologous recombination in rice.The mutable flecked, speckled, r-1, and purple alleles in I. nil were caused by insertions of Tpn1 and its relatives in the En/Spm superfamily, Tpn2, Tpn3, and Tpn4, into the genes for anthocyanin coloration in wers, i.e., DFR-B. CHI, CHS-D, and InNHX1, respectively. Similarly, the flaked and pink mutants of I. purpurea have distantly related elements, Tip1OO and Tip201, in the Ac/Ds superfamily inserted into the CHS-D and F3'H genes, respectively. Concerning gene targeting in rice, an important staple food and a model plant for other cereal species, we have developed a large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection and succeeded a reproducible targeting of the Waxy gene by homologous recombination without concomitant occurrence of undesirable ectopic events.

高等植物中主要的基于DNA的控制转座因子可分为三大类：Ac/Ds（或hAT）超家族、En/Spm（或CACTA）超家族和Mu家族。作为花卉植物，普通牵牛花的自发突变体（I。purpurea），而日本牵牛花的各种自发突变体（I.无），显示出许多不同的花色。我们正试图确定这些自发突变体，包括可变等位基因赋予杂色花。此外，我们还尝试了利用同源重组技术在水稻中进行基因打靶，将I. nil是由Tpn 1及其在En/Spm超家族中的亲属Tpn 2、Tpn 3和Tpn 4插入wers中的花色素苷着色基因引起的，即，DFR-B。CH 1、CHS-D和InNHX 1。同样，I. Purpurea在Ac/Ds超家族中有两个亲缘关系较远的元件Tip 100和Tip 201，分别插入到CHS-D和F3 ′ H基因中。关于水稻，一个重要的主食和其他谷物物种的模式植物中的基因靶向，我们已经开发了一个大规模的农杆菌介导的转化程序，具有强大的正-负选择，并成功地通过同源重组的WESTERN基因的可重复的靶向没有伴随发生不良的异位事件。