New mutagenesis approaches to study gene function in crop
New mutagenesis approaches to study gene function in crop
批准号:
22658004
负责人:
IIDA Shigeru
金额:
$2.29万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
为了有效地研究基因功能,我们试图发展反向遗传学方法,即通过同源重组介导的基因打靶和利用内源nDart1相关DNA转座子的基因标记来条件敲除和产生细微突变。我们还对通过基因打靶和标记分离到的插入突变体进行了鉴定。
英文摘要
To characterize gene function effectively, we tried to develop reverse genetic approaches, namely conditional knock-out and generation of subtle mutations by homologous recombination-mediated gene targeting and gene tagging employing endogenous nDart1-related DNA transposons. We also characterized insertion mutants isolated by gene targeting and tagging.
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イオンビーム処理によるイネのDNA トランスポゾン Dart の活性化
离子束处理激活水稻 DNA 转座子 Dart
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[前川雅彦, 西村秀希, 氷見英子, 飯田滋, 栂根一夫]
通讯作者:
栂根一夫
イネ内在性 DNA トランスポゾン nDart の挿入部位指向性と遺伝子タギングへの応用
水稻内源DNA转座子nDart的插入位点靶向及其在基因标记中的应用
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[栂根一夫, 高木恭子, 前川雅彦, 飯田滋]
通讯作者:
飯田滋
DOI:
10.1266/ggs.86.215
发表时间:
2011-06
期刊:
Genes & genetic systems
影响因子:
1.1
作者:
[Mika Hayashi-Tsugane;M. Maekawa;Hirokazu Kobayashi;S. Iida;Kazuo Tsugane]
通讯作者:
Mika Hayashi-Tsugane;M. Maekawa;Hirokazu Kobayashi;S. Iida;Kazuo Tsugane
Identification of the transposon-tagged gene essential for chloloplast biogenesis in rice
水稻叶绿体生物发生必需的转座子标记基因的鉴定
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[M. Hayashi-Tsugane, H. Takahara, N. Ahmed, E. Himi, K. Takagi, S. Iida, M.Maekawa and K. Tsugane]
通讯作者:
M.Maekawa and K. Tsugane
Effective contribution of the nDart transposon-tagging system to rice functional genomics. K. V. Urbano ed., Advances in Genetics Research 4
nDart 转座子标签系统对水稻功能基因组学的有效贡献。
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[M. Maekawa, K. Tsugane and S. Iida]
通讯作者:
K. Tsugane and S. Iida
共 12 条
Reverse genetics and recombination
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批准号:22370001
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项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$12.06万
-
财政年份:2010
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负责人:IIDA Shigeru
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依托单位:
Genetics and epigenetics in higher plants
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批准号:17207002
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$32.28万
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财政年份:2005
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负责人:IIDA Shigeru
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依托单位:
The genome dynamics and function in higher plants
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批准号:14204075
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$28.62万
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财政年份:2002
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负责人:IIDA Shigeru
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依托单位:
Genome rearrangements and gene regulation in higher plants
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批准号:12440213
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.86万
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财政年份:2000
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负责人:IIDA Shigeru
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依托单位:
Transposition and gene regulation of DNA transposable elements in plants
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批准号:10216208
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$29.57万
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财政年份:1998
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负责人:IIDA Shigeru
-
依托单位:
New methods for gene identification based on genomic analysis and their application for molecular breeding in plants.
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批准号:10554048
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$8.51万
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财政年份:1998
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负责人:IIDA Shigeru
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依托单位:
Comprehensive Study on Deer and Wild boar for Non-wood Forest Products
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批准号:09306009
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项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$15.42万
-
财政年份:1997
-
负责人:IIDA Shigeru
-
依托单位:
Identification of new transposable elements in the mutable alleles for flower variegation in the Japanese and common morning glories and transposition of the maize Ac/Ds elements in transgenic rice plants.
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批准号:08454261
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$5.12万
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财政年份:1996
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负责人:IIDA Shigeru
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依托单位:
Search for an unknown mobile genetic element controlling expression of genes in phenylpropanoid biosynthesis
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批准号:03807150
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1991
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负责人:IIDA Shigeru
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依托单位:
DNA Rearrangements Caused by Non-Homologous Recombinatin and Gene Expression
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批准号:01480540
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.29万
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财政年份:1989
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负责人:IIDA Shigeru
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依托单位:
海外基金