Defining nuclear components of the PAMP-triggered and Effector-triggered immune responses mediated by AtWRKY transcription factors

定义 AtWRKY 转录因子介导的 PAMP 触发和效应器触发免疫反应的核成分

• 批准号: 49762463
• 负责人: Dr. Imre Somssich
• 金额: --
• 依托单位: Abteilung Pflanze-Mikroben Interaktionen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/49762463?language=en
• 关键词: Defining; nuclear; components; PAMP; triggered

Plant immune responses are associated with comprehensive transcriptional reprogramming. Recently, genetic evidence directly proving the significance of WRKY transcription factors as positive and negative regulators of disease resistance has accumulated. Importantly, WRKY proteins were shown to act as interfaces physically linking two distinct defense signaling pathways and to be functionally connected forming a transcriptional network composed of positive and negative feedback loops and feed-forward modules. Here we aim at identifying nuclear components of the defense signaling transduction pathways involving WRKY transcription factors. We plan to test the possible interaction of NBS-LRR type immune receptors with WRKY transcription factors that have been shown to be involved in PAMP-triggered and effectortriggered immunity. Furthermore, we want to identify potential interactors and direct target genes of AtWRKY33, a WRKY factor acting downstream of the MAP kinase MPK4 in basal defense and systemic acquired resistance. These studies should provide valuable insights on how WRKY factors function as nodes to integrate distinct pathogen-triggered host defense signals.

植物免疫反应与全面的转录重编程有关。近年来，越来越多的遗传证据直接证明WRKY转录因子作为抗病性的正、负调节因子的重要性。重要的是，WRKY蛋白被证明作为物理连接两种不同防御信号通路的界面，并在功能上连接，形成由正反馈环和负反馈环以及前馈模块组成的转录网络。在这里，我们的目的是确定核组件的防御信号转导通路涉及WRKY转录因子。我们计划测试NBS-LRR型免疫受体与WRKY转录因子的可能相互作用，这些转录因子已被证明参与PAMP触发和效应物触发的免疫。此外，我们希望确定潜在的相互作用和AtWRKY 33的直接靶基因，一个WRKY因子在基础防御和系统获得性抗性中作用于MAP激酶MPK 4的下游。这些研究将为WRKY因子如何作为节点整合不同的病原体触发的宿主防御信号提供有价值的见解。