Functional dissection of the WRKY family of transcription factors involved in senecence and pathogen defense
参与衰老和病原体防御的 WRKY 转录因子家族的功能剖析
基本信息
- 批准号:5352072
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2001
- 资助国家:德国
- 起止时间:2000-12-31 至 2007-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
WRKY proteins are a new family of zinc-finger type transcription factors found exclusively in plants. In Arabidopsis thaliana, this large gene family consists of 75 members. The plantspecific existence of this superfamily suggests that WRKY factors are involved in several unique processes required for plant developments and/or for the integration of environmental cues. Although still poorly studied, WRKY Proteins appear to play an important regulatory role under stress-related conditions particularly during defense against phytopathogenic microbes. Aim of this project is to use defined Arabidopsis WRKY "knock-out" and inducible WRKY overexpressor lines for comparative protein profiling studies with wild-type plants under several defined stress-related conditions including senescence. Peptides differentially appearing in such profiles will be identified by MALDI-MS/MALDI-TOF. In addition, we plan to identify WRKY interacting partners with the help of epitope-tagged WRKY proteins and by the use of the split-Ubiquitin-method or the Ras-Recruitment-System.
WRKY蛋白是一个新的锌指型转录因子家族,仅在植物中发现。在拟南芥中,这个大基因家族由75个成员组成。该超家族的植物特异性存在表明WRKY因子参与了植物发育和/或环境线索整合所需的几个独特过程。尽管目前对WRKY蛋白的研究还不多,但WRKY蛋白在逆境条件下,特别是在防御植物病原微生物的过程中,似乎起着重要的调节作用。本项目的目的是使用定义的拟南芥WRKY“敲除”和诱导WRKY过表达线的比较蛋白质谱研究与野生型植物在几个定义的压力相关的条件下,包括衰老。将通过MALDI-MS/MALDI-TOF鉴定在这些图谱中差异出现的肽。此外,我们计划确定WRKY相互作用的合作伙伴的帮助下,表位标记的WRKY蛋白和使用的分裂泛素方法或Ras招募系统。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Dr. Imre Somssich其他文献
Dr. Imre Somssich的其他文献
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{{ truncateString('Dr. Imre Somssich', 18)}}的其他基金
Defining nuclear components of the PAMP-triggered and Effector-triggered immune responses mediated by AtWRKY transcription factors
定义 AtWRKY 转录因子介导的 PAMP 触发和效应器触发免疫反应的核成分
- 批准号:
49762463 - 财政年份:2007
- 资助金额:
-- - 项目类别:
Research Grants
Targeted disruption of the WRKY gene family encoding zinc-finger type transcription factors in the moss Physcomitrella patens
定向破坏小立碗藓中编码锌指型转录因子的 WRKY 基因家族
- 批准号:
5218890 - 财政年份:1999
- 资助金额:
-- - 项目类别:
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