Studies on signal and mechanism studies on tuber-and flower bud-formation

块茎和花芽形成的信号及机制研究

• 批准号: 12045201
• 负责人: YOSHIHARA Teruhiko
• 金额: $ 9.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12045201/
• 关键词: tuber formation; flower bud formation; lipoxygenase; light; temperature; セオブロキシド; リポキシゲナーゼ; リノレン酸; マイクロチューバー; バレイショ; アサガオ

Theobroxide stimulates the activity of lipoxygenase and induces potato tuber formation and flower bud formation of morning glory under even non-inducing condition, long days.The effects of light and temperature on the activity of lipoxygenase on flower bud formation and tuber were investigated, because flower bud formation and tuber formation are strongly related to the surrounding factors such as light and temperature.The effect of light on lipoxygenase activity was investigated.In leaves of morning glory of dark durations (0, 15, 30 and 60 min.), which were switched from the light irradiation, lipoxygenase activity was increased until 30 min.and then started to decrease.However, lipoxygenase activity in plants switched from dark to light condition showed no significant alteration.The temperature effects on the flower bud of morning glory and the tuber formation of potato were also exarnined.Flower buds of morning glory were produced both at 25°C and 30°Cand lipoxygenase activities were also high at these two temperature conditions.At 35°C, no flower bud was formed and lipoxygenase activity was low.Potato was cultivated at various temperature conditions ( 15, 20, 25 and 30°C).The greatest tuber yield was observed at 20°C and the highest lipoxygenase activity was measured at 15°C.These date show that lipoxygenase of plants are influenced by the light and temperature and stimulate tuber formation and flower bud formation.

由于花芽形成和块茎形成与光照、温度等环境因素密切相关，因此研究了光照和温度对马铃薯块茎形成和向日葵花芽形成的影响。研究了光照对0、15、30和60min暗时间(0、15、30和60min)的牵牛花叶片中脂肪氧合酶活性的影响。在从光照转换到30min时，脂肪氧合酶活性一直升高到30min，然后开始下降。然而，随着光照时间的延长，脂肪氧合酶的活性逐渐降低，但随着光照时间的延长，脂肪氧合酶的活性逐渐降低不同温度条件(15℃、20℃、25℃、30℃)对马铃薯块茎形成和块茎形成的影响较大。25℃和30℃条件下均能产生牵牛花花蕾，在这两种温度条件下都有较高的脂氧合酶活性。35℃时不形成花蕾，脂肪氧合酶活性较低。马铃薯在不同温度下(15℃、20℃、25℃和30℃)培养，块茎产量最高，15℃时脂肪氧合酶活性最高。

项目成果

Hideyuki Matsuura: "Occurrence of 11-Hydroxyjasmonic Acid Glucoside in Leaflets of Potato Plants (Solanum tuberosum L.)"Biosci.Biotechnol.Biochem. 65. 378-382 (2001)

Hideyuki Matsuura：“马铃薯植物（Solanum tuberosum L.）小叶中 11-羟基茉莉酸葡萄糖苷的出现”Biosci.Biotechnol.Biochem。

H.Matsuura: "Occurrence of 11-hydroxyjasmonic Acid Glucoside in Leaflets of Potato Plants"Biosci.Biotechnol.Biochem.. 65-2. 378-382 (2001)

H.Matsuura：“马铃薯植物小叶中 11-羟基茉莉酸葡萄糖苷的出现”Biosci.Biotechnol.Biochem.. 65-2。

H.Matsuura: "Qualitative and Quantitative Analysis of Endogenous Jasmonoids in Potato Plant"Biosci.Biotechnol.Biochem.. 64-11. 2380-2387 (2000)

H.Matsuura：“马铃薯植物内源茉莉酮类化合物的定性和定量分析”Biosci.Biotechnol.Biochem.. 64-11。

吉原照彦: "ジャスモン酸の生理と化学"農業および園芸. 75-10. 1091-1094 (2000)

Teruhiko Yoshihara：“茉莉酸的生理学和化学”农业和园艺75-1094（2000）。

Q.Yang: "Potato microtuber inducing hydroxylasiodiplodins from Lasiodiplodia theobromae"Phytochemistry. 54. 489-494 (2000)

Q.Yang：“马铃薯微块茎诱导来自可可二倍体的羟基西二倍体”植物化学。