Research of development on the regulator of flower-bud formation

花芽形成调控因子的开发研究

• 批准号: 13356002
• 负责人: YOSHIHARA Teruhiko
• 金额: $ 28.04万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13356002/
• 关键词: flower bud formation; theobroxide; Lasiodiplodia theobromae; regulation of flower bud formation

1.Flower bud formation by theobroxide1mM theobtoxide was sprayed to the test plants. Short day plants (rice, strawberry, soybean and poinsettia) produced flower bud. But there were difference of the effect between cultivars of strawberry and soybean.2.Potato tuber formation by theobroxideThe field experiment of theobroxide treatment showed the increase of the yield 20% over non treatment. And theobroxide treatment induced the resistance against potato diseases.3.Inhibition of the bolting of spinach by theobroxideTheobroxide treatment inhibited the bolting of spinach.4.Mechanism of theobroxideBy the experiment using a lipoxygenase inhibitor, the function of theobroxide is revealed that theobroxide stimulates the activity of a lipoxygenase. The activated lipoxygenase produces the potato tuber inducing stimulus tuberonic acid glucoside and flower inducing factor (?).5.Application of the patentTwo patents on the production method of theobroxide were applied. The methods are fermentation and organic synthesis.

1.对供试植株喷施1 mM茶碱形成的花芽。短日照植物(水稻、草莓、大豆和一品红)会产生花蕾。2.茶氧化物对马铃薯块茎形成的影响大田试验表明，茶氧化物处理比不处理增产20%。3.茶树氧化物对菠菜抽苔的抑制作用。4.茶树氧化物的作用机理通过使用脂氧合酶抑制剂的实验，揭示了茶树氧化物刺激脂肪加氧酶活性的作用。活化的脂肪氧合酶产生马铃薯块茎诱导刺激的块茎糖苷和开花诱导因子(？)。5.专利申请申请了两项关于可可醚生产方法的专利。方法有发酵法和有机合成法。

项目成果

Xiquan Gao, Qing Yang, Chisata Minami, Hideyuki Matsuura, Atsuo Kimura, Teruhiko Yoshihara: "Inhibitory effect of salicylhydroxamic acid on Theobroxide-induced potato tuber formation"Plant Science. 165. 993-999 (2003)

高希泉、杨清、南千萨塔、松浦英幸、木村厚男、吉原照彦：“水杨基异羟肟酸对可可醇诱导的马铃薯块茎形成的抑制作用”植物科学。

Hideyuki Matsuura: "Metabolism of Deuferium-labeled Jasmonic Acid and OPC 8:0 in the potato plant (Solanum tuber sumL.)"Biosci.Biotechnol.Biochem. 67・9. 1903-1907 (2003)

Hideyuki Matsuura：“马铃薯植物中氘标记的茉莉酸和 OPC 8:0 的代谢”Biosci.Biotechnol.Biochem. 67・9 (2003)。

Hideyuki Matsuura: "Occurrence of 11-Hydroxyjasmonic Acid Glucoside in Leaflets of Potato Plants (Solanum tuberosum L.)"Biosci.Biotechnol.Biochem. 65. 378-382 (2001)

Hideyuki Matsuura：“马铃薯植物（Solanum tuberosum L.）小叶中 11-羟基茉莉酸葡萄糖苷的出现”Biosci.Biotechnol.Biochem。

Qing Yang, Hideaki Toshima, Teruhiko Yoshihara: "Synthesis of β-resorcylic acid derivatives, novel Potato microtuber inducing substances isolated from Lasiodiplodia theobromae"Tetrahedron. 57. 5377-5384 (2001)

Qing Yang、Hideaki Toshima、Teruhiko Yoshihara：“β-间苯二酚酸衍生物的合成，从可可二孢菌中分离出的新型马铃薯微型块茎诱导物质”Tetrahedron 57. 5377-5384 (2001)。

Qing Yang: "Syntheses of β-resocylic acid derivatives, novel potato microtuber inducing substances isolated fromLasiodiplodia theobromae"Tetrahedron. 57. 5377-5384 (2001)

杨庆：“从可可毛二孢中分离出的新型马铃薯微型块茎诱导物质β-间苯二甲酸衍生物的合成”Tetrahedron 57. 5377-5384 (2001)