权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Evolution and Diversity in the system of development and differentiation

发育和分化系统中的进化和多样性

基本信息

批准号：
12202005
负责人：
GOJOBORI Takashi
金额：
$ 97.92万
依托单位：
National Institute of Genetics
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2004
项目状态：
已结题

项目摘要

(1) Evolution and Developmental mechanism in Nervous systemTo understand when and how the structural brain emerged, we analyzed the central nervous system of a lower invertebrate, planarian. We conducted a large-scale screening of the head part-specific genes in the planarian by constructing a cDNA microarray. As a result, 205 genes with head part-specific spikes were found, which included some essential genes in the vertebrate nervous system. The expression patterns of the top 30 genes showing the strongest spikes implied that the planarian brain has undergone functional regionalization.As a result of homology searches, we found that 116 clones had significant similarity to known genes related to the nervous system. Interestingly, we found that 〜30% of planarian nervous system-related genes had homologous sequences in Arabidopsis and yeast, which do not possess a nervous system. This implies that the origin of nervous system-related genes greatly predated the emergence of the nervous system, and that these genes might have been recruited toward the nervous system.(2) Evolution and Diversity in the system of development and differentiationTo investigate the diversification of cichlid jaw morphology at the genetic level, we used a DNA chip to compare gene expression levels between closely related cichlid fishes. This analysis indicated that the expression of some genes differed in the larvae of two cichlid species. One such clone encodes a new astacin family metalloproteinase. Because some astacin family proteins contribute to morphogenesis in animals, CiMP1 is expected to participate in species-specific head morphogenesis in cichlids.In Cichlids, here, we provide an example in which alternative splicing may play a role in mate choice, leading to cichlid speciation through diversification of gene function by production of multiple mRNAs from a single gene.

(1)神经系统的演化与发育机制为了了解结构脑是何时以及如何出现的，我们分析了一种低等无脊椎动物--真涡虫的中枢神经系统。我们通过构建cDNA微阵列，对真涡虫头部特异基因进行了大规模筛选。结果发现205个基因具有头部特异性刺突，其中包括脊椎动物神经系统的一些必需基因。前30个基因的表达模式显示出最强的尖峰，这意味着涡虫大脑已经经历了功能区域化。作为同源性搜索的结果，我们发现116个克隆与已知的神经系统相关基因有显著的相似性。有趣的是，我们发现大约30%的涡虫神经系统相关基因在拟南芥和酵母中具有同源序列，而拟南芥和酵母并不具有神经系统。这意味着神经系统相关基因的起源大大早于神经系统的出现，并且这些基因可能已经被招募到神经系统中。(2)发育分化系统的进化与多样性为了在遗传水平上研究慈鲷科鱼类颌骨形态的多样性，我们使用DNA芯片比较了近缘慈鲷科鱼类之间的基因表达水平。这一分析表明，一些基因的表达在两种慈鲷的幼虫不同。一个这样的克隆编码一个新的astacin家族金属蛋白酶。由于一些astacin家族蛋白有助于动物的形态发生，CiMP 1预计将参与物种特异性的头部形态发生在慈鲷科，在这里，我们提供了一个例子，其中选择性剪接可能在配偶选择中发挥作用，导致慈鲷物种通过多样化的基因功能，从一个单一的基因产生多个mRNA。

项目成果

期刊论文数量（334）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Microarray analysis of embryonic retinoic acid target genes in the ascidian Ciona intestinalis.

海鞘胚胎视黄酸靶基因的微阵列分析。

DOI：
发表时间：
2003
期刊：
Development, Growth & Differentiation 45
影响因子：
0
作者：
Ishibashi;T.
通讯作者：
T.

Biased biological functions of horizontally transferred genes on 324,653 open reading frames of 116 prokaryotic complete genomes

116个原核生物完整基因组的324,653个开放阅读框上水平转移基因的生物学功能偏差

DOI：
发表时间：
2004
期刊：
Nature Genetics 36
影响因子：
0
作者：
Nakamura;Y.
通讯作者：
Y.

Origin and evolutionary process of the CNS elucidated by comparative genomics analysis of planarian ESTs

DOI：
10.1073/pnas.1332513100
发表时间：
2003-06-24
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Mineta, K;Nakazawa, M;Gojobori, T
通讯作者：
Gojobori, T

The origin and evolution of porcine reproductive and respiratory

猪生殖和呼吸系统的起源和进化