Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials
Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials
批准号:
13031024
负责人:
NAKAMURA Satoshi
金额:
$33.28万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2003
中文摘要
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英文摘要
The objective of this research is to elucidate the importance of non-covalent bonds in proteins on tolerances towards extreme environments. A 2Fe-2S feredoxin from triangular disc-shaped extremely halophilic archaeon Haloarcula japonica was investigated. It seemed that the additional amino terminal region of H. japonica Fd was importantfor halotolerancy. The thermostability of 3-isopropylmalate dehydrogenase of moderate facultative thermophile Bacillus coagulans was improved by amino acid substitutions at the subunit-subunit interface and/or a loop region.Furthermore, the function of chitin-binding domain of a multi-domain chitinase from alkaliphilic Bacillus sp. J813 was elucidated.
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R.Yatsunami et al.: "A Novel uhitosanase trom bacillus sp. Strain K17 : Gene Cloning and Expression in Escherichia coli"Nucleic Acids Res.Suppl.. 2. 227-228 (2002)
R.Yatsunami 等人:“芽孢杆菌属菌株 K17 中的新型 uhitosanase:基因克隆和在大肠杆菌中的表达”Nucleic Acids Res.Suppl.. 2. 227-228 (2002)
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M.Inami: "Directed Evolution of Xylanase J form Alkaliphilic Bacillus sp. Strain 41M-1"Nucleic Acids Res. Suppl.. 3. 315-316 (2003)
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K.Endo et al.: "Molecular Cloning of a Chitinase Gene from Newly Isolated Alkaliphilic Nocardiopsis sp. Strain F96"Extremophiles. (In press). (2003)
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共 30 条
Characterizaition and functional modification of GH family 18 chitinases from alkaliphilic bacteria
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Impact of endothelin-1 on microcirculatory disturbance after partial hepatectomy under ischemia/reperfusion in thioacetamide-induced cirrhotic rats
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Molecular Anatomy of Polysaccharide-degrading Enzymes from Extremophiles
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Biomimetic control on ceramics surfaces by polarization induction
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Structure and function of halotolerant metalloprotein from extremely halophilic archaeon
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Structure and Functions of Polysaccharide-degrading Enzyme with High Activity under Extreme Environments
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A new diagnostic method of cancer with Positron Emission Tomography
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Studies on the Triangular-shaped Extremely Halophilic Archaeon
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Experimental Study on the Functional Changes of Hepatic Reticuloendothelial System during Acute Liver Rejection.
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海外基金