Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials

极端条件下生物分子间疏水相互作用在强相关软材料中的应用

• 批准号: 13031024
• 负责人: NAKAMURA Satoshi
• 金额: $ 33.28万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13031024/
• 关键词: Bioengineering; Biotechnology; Softmaterial; Protein; Extreme environment

The objective of this research is to elucidate the importance of non-covalent bonds in proteins on tolerances towards extreme environments. A 2Fe-2S feredoxin from triangular disc-shaped extremely halophilic archaeon Haloarcula japonica was investigated. It seemed that the additional amino terminal region of H. japonica Fd was importantfor halotolerancy. The thermostability of 3-isopropylmalate dehydrogenase of moderate facultative thermophile Bacillus coagulans was improved by amino acid substitutions at the subunit-subunit interface and/or a loop region.Furthermore, the function of chitin-binding domain of a multi-domain chitinase from alkaliphilic Bacillus sp. J813 was elucidated.

本研究的目的是阐明蛋白质中非共价键对极端环境耐受性的重要性。研究了三角盘状极端嗜盐古菌Haloarcula japonica中的2Fe-2S铁氧还蛋白。H. japonica Fd对耐盐性有重要作用。通过对中等兼性嗜热芽孢杆菌凝结芽孢杆菌（Bacillus coagulans）3-异丙基苹果酸脱氢酶（3-isopropyl malate dehydrogenase，3-IDH）亚基-亚基界面和/或环区进行氨基酸取代，提高了其热稳定性，并对嗜碱芽孢杆菌J813多结构域几丁质酶的几丁质结合结构域的功能进行了初步研究。

项目成果

S.Nakamura: "Structure and Function of a Multidomain Alkaline Xylanase from Alkalinhilic Bacillus sp. Strain 41M-1"Catal.Survey Asia. 7. 157-164 (2003)

S.Nakamura：“嗜碱性芽孢杆菌菌株 41M-1 的多域碱性木聚糖酶的结构和功能”Catal.Survey Asia。

R.Yatsunami et al.: "A Novel uhitosanase trom bacillus sp. Strain K17 : Gene Cloning and Expression in Escherichia coli"Nucleic Acids Res.Suppl.. 2. 227-228 (2002)

R.Yatsunami 等人：“芽孢杆菌属菌株 K17 中的新型 uhitosanase：基因克隆和在大肠杆菌中的表达”Nucleic Acids Res.Suppl.. 2. 227-228 (2002)

M.Inami: "Directed Evolution of Xylanase J form Alkaliphilic Bacillus sp. Strain 41M-1"Nucleic Acids Res. Suppl.. 3. 315-316 (2003)

M.Inami：“嗜碱性芽孢杆菌菌株 41M-1 中木聚糖酶 J 的定向进化”核酸研究。

八波利恵ほか: "生体内分子の自己修復"科学と教育. 49. 122-125 (2001)

Rie Yamba 等人：“生物分子的自我修复”科学与教育 49. 122-125 (2001)

K.Endo et al.: "Molecular Cloning of a Chitinase Gene from Newly Isolated Alkaliphilic Nocardiopsis sp. Strain F96"Extremophiles. (In press). (2003)

K.Endo 等人：“从新分离的嗜碱诺卡氏菌 F96 菌株中进行几丁质酶基因的分子克隆”。