Study of diagnosis and spatial epidemiology on paragonimiasis in Lao PDR.

老挝肺吸虫病诊断和空间流行病学研究。

• 批准号: 16390188
• 负责人: NAKAMURA Satoshi
• 金额: $ 8.13万
• 依托单位: Research Institute, International Medical Centre of Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390188/
• 关键词: Infectious disease; Social Medicien; Parasitology; Applied zoology; Ecology; Geographic Information System; 応用動物; ラオス; 肺吸虫症; 診断; 空間疫学; 中間宿主

In spite of lack of information on paragonimiasis in Laos since 1973, eight districts in 5 provinces; Attopeu, Champasak, Savannakhet, Vientiane, and Luangnamtha, which exist and/or possible risk of the disease were clarified through our research. Around 700 metacercariae were collected from 2 species of the river crabs (7 Potamon lipkei and 5 Chulathelphusa brandti) obtained in Hinheup district which was especially investigated as a dense infested area in Vientiane province, where is 80 km north from Vientiane Capital in Lao PDR. Though the numbers were smaller, Paragonimus heterotremus, a known causative species of human parogonimiasis in Indochina region, and P. westermani were identified at the metacercariae population. On the major population, 2 unreported Paragonimus species of harinastai and bankokensis were identified and clarified using adult worms obtained from experimental infections of dogs in the laboratory. Among various experimental animals, adult worms of P. harinastai never survive after 3 month of the experimental infections in rats, which was demonstrated for the first time. Furthermore, dog is a suitable host for P. bankokensis was confirmed by recovery (80%) of the adult worms at the experimental infections. And the established PCR technology was used. To identify the actual causative species of human paragonimiasis in Hinheup, 6 samples of the parasite eggs from the sputa of dwellers were collected, and the established PCR technique; using primers of species specific region (ITS2) of the 4 parasites, was performed. All these samples resulted in P. heterotremus, thus the causative parasite of human paragonimiasis was strongly suggested as this species in the region.

尽管自1973年以来老挝缺乏关于并殖吸虫病的信息，但通过我们的研究，查明了5个省的8个地区：阿托帕乌、尚巴萨克、萨凡纳赫特、万象和朗南塔，这些地区存在和/或可能存在并殖吸虫病的风险。在老挝首都万象以北80公里的万象省，特别是调查了兴海普地区采集的2种河蟹(7种河蟹和5种布氏绒蟹)，共采集囊虫约700个。虽然数量较少，但在囊虫种群中发现了已知的引起印度支那地区人类副殖吸虫病的异型并殖吸虫和卫氏并殖吸虫。在主要种群中，利用实验室实验感染犬的成虫，鉴定和澄清了2种未报告的并殖吸虫种：哈里纳斯泰和班戈氏并殖吸虫。在各种实验动物中，哈氏肺吸虫成虫在大鼠实验感染3个月后不能存活，这是首次证实的。此外，实验感染的成虫回收率为80%，证明狗是班氏肺吸虫的适宜宿主。并采用已建立的聚合酶链式反应技术。为鉴定兴和浩特地区人体并殖吸虫病的真实病原种类，采集了6份居民痰中的寄生虫卵，建立了4种寄生虫的种特异区(ITS2)的聚合酶链式反应(PCR)技术。所有样本均检出异源并殖吸虫，因此该地区人并殖吸虫病的致病原虫可能是该种。

项目成果

Distribution of Paragonimus species in the crab host at Vientiane Capital in Lao People's Democratic Republic

老挝人民民主共和国首都万象蟹寄主中并殖吸虫属的分布

• 期刊: Parasitology (発表予定)
• 作者: Satoshi Nakamura;Shigehisa Habe;et al.
• 通讯作者: et al.

ラオスにおける人肺吸虫症の原因種の同定(学会発表)

老挝人类肺吸虫病病原体的鉴定（会议报告）

• 发表时间: 2006
• 期刊: 第47回日本熱帯医学会総会抄録集(熱帯医学特別号) 34
• 作者: 波部重久;八尋伸一郎;中村 哲
• 通讯作者: 中村 哲

Identification of human Paragonimus species collected from Laotian patients.

从老挝患者身上采集的人类并殖吸虫种类的鉴定。

• 发表时间: 2006
• 期刊: 11^<th> International Congress of Parasitology, Glasgow, UK : Abstract book (CD-R)
• 作者: S.Yahiro;S.Habe;S.Nakamura et al.
• 通讯作者: S.Nakamura et al.

ラオスにおける肺吸虫症

老挝并殖吸虫病

• 发表时间: 2005
• 期刊: Tropical Medicine and Health 33(増刊号)
• 作者: 波部重久;中村 哲;他
• 通讯作者: 他

秋道智彌 編 図説 メコンの世界

富宫秋道编 湄公河世界插画

• 发表时间: 2007
• 作者: Satoshi Nakamura;Shigehisa Habe;et al.;中村 哲
• 通讯作者: 中村 哲