Creation of novel matrix for cell culture using CBMand chitin
Creation of novel matrix for cell culture using CBMand chitin
批准号:
21500423
负责人:
NAKAMURA Satoshi
金额:
$2.83万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
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英文摘要
Chitinase J from alkaliphilic Bacillus sp. contained a catalytic domain, a fibronectin type III-like domain(FnIIID) and a chitin-binding domain(ChBD). A peptide sequence Arg-Gly-Asp(RGD) sequence locates within fibronectin, an extracellular matrix molecule, and interacts with cells via a cell surface receptor called integrin. Several kinds of RGD-fused FnIIID/ChBD and ChBD proteins were prepared and characterized. The fusion proteins indicated chitin-binding, integrin-binding and cell adhesive activities.
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Analysis of functional domains and improvement of alkaliphily of an alkaline xylanase on the basis of its three-dimensional structure
基于三维结构的碱性木聚糖酶功能域分析及碱性改进
DOI:
--
发表时间:
2009
期刊:
J. Appl. Glycosci.
影响因子:
--
作者:
[H. Umemoto, R. Yazawa, J. Takakura, R. Yatsunami, T. Fukui and S. Nakamura]
通讯作者:
T. Fukui and S. Nakamura
A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliohilic Bacillus sp. strain 41M-1
来自嗜碱性芽孢杆菌属的碱性木聚糖酶的钙依赖性木聚糖结合域。
DOI:
--
发表时间:
2011
期刊:
Biosci.Biotechnol.Biochem.
影响因子:
--
作者:
[Risa Yazawa, 他]
通讯作者:
他
Additional carbohydrate-binding modules enhance the insoluble substrate hydrolytic activity of beta-1, 3-glucanase from alkaliphilic Nocardiopsis sp. F96
额外的碳水化合物结合模块增强了来自嗜碱性诺卡氏菌属的 β-1, 3-葡聚糖酶的不溶性底物水解活性。
DOI:
--
发表时间:
2009
期刊:
Biosci. Biotechnol. Biochem.
影响因子:
--
作者:
[N. Koizumi, S. Masuda, K. Maeda, Y. Isoda, R. Yatsunami, T. Fukui and S. Nakamura]
通讯作者:
T. Fukui and S. Nakamura
CBMファミリー36キシラン結合ドメインの基質結合機構と2価金属イオンの役割
CBM家族36木聚糖结合域的底物结合机制及二价金属离子的作用
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[谷澤里沙, 他]
通讯作者:
他
ソフトマテリアルの応用展開(西敏夫・編),シーエムシー,大阪
软材料的应用开发(Toshio Nishi,编辑),CMC,大阪
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Young-Ho Lee, 他, 三原久和,中村聡,小畠英理]
通讯作者:
三原久和,中村聡,小畠英理
共 22 条
Characterizaition and functional modification of GH family 18 chitinases from alkaliphilic bacteria
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Study on Information Clouding
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International cooperation research concerning water-borne diseases in relocated people and the development of related risk management techniques
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Study of diagnosis and spatial epidemiology on paragonimiasis in Lao PDR.
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Development of osteoconductive materials using ion conductive ceramics
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Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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财政年份:2001
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负责人:NAKAMURA Satoshi
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Impact of endothelin-1 on microcirculatory disturbance after partial hepatectomy under ischemia/reperfusion in thioacetamide-induced cirrhotic rats
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资助金额:$1.15万
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依托单位:
Molecular Anatomy of Polysaccharide-degrading Enzymes from Extremophiles
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批准号:12650783
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项目类别:Grant-in-Aid for Scientific Research (C)
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财政年份:2000
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依托单位:
Biomimetic control on ceramics surfaces by polarization induction
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项目类别:Grant-in-Aid for Scientific Research (C)
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财政年份:1999
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依托单位:
Mechanism of vulnerability to endotoxin of regenerating liver and its treatment
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财政年份:1998
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依托单位:
Structure and function of halotolerant metalloprotein from extremely halophilic archaeon
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财政年份:1997
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Structure and Functions of Polysaccharide-degrading Enzyme with High Activity under Extreme Environments
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.6万
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财政年份:1995
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A new diagnostic method of cancer with Positron Emission Tomography
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批准号:06454364
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资助金额:$4.29万
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财政年份:1994
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Studies on the Triangular-shaped Extremely Halophilic Archaeon
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批准号:05650792
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1993
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Experimental Study on the Functional Changes of Hepatic Reticuloendothelial System during Acute Liver Rejection.
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批准号:02454301
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$1.28万
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财政年份:1990
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Light dosimetry of liver tissues and hepatic tumors in rats and human. --Experimental study and its application--
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批准号:62570563
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.15万
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财政年份:1987
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负责人:NAKAMURA Satoshi
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依托单位:
国内基金
海外基金
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基于理性设计融合CBM修饰壳聚糖酶结构及其产物特异性调控机制研究
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