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Study of cell morphogenesis that is coupled with cell division cycle

与细胞分裂周期耦合的细胞形态发生研究

基本信息

批准号：
16026205
负责人：
OHYA Yoshikazu
金额：
$ 14.78万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16026205/
关键词：
cell division cycle Saccharomyces cerevisiae cell wall checkpoint dynactin cell morphology

项目摘要

The coordination of cell cycle events during cell proliferation is attained, in part, through surveillance systems. In the budding yeast Saccharomyces cerevisiae, cell cycle progression is restrictively regulated and some events during cell cycle are regulated by the checkpoints monitoring DNA damage, DNA replication, integrity of the spindle, spindle positioning and cell morphogenesis. It still remained unclear whether all of the checkpoint controls have been identified.We found that a novel checkpoint exists to couple cell wall remodeling with spindle formation. fksl mutants defective in 1,3-β-glucan synthase activity failed to form a mature bud. Surprisingly, these cells arrested with post-replicative DNA but quite low level of C1b2p, and without forming bipolar spindles. We reported that wadi (wall-checkpoint defective) mutation that abolished this arrest caused the accumulation of C1b2p and CLB2 mRNA and leaded to formation of bipolar spindles despite glucan-synthesis perturbation. These results indicate the existence of a novel checkpoint mechanism to coordinate entry into mitosis, and suggest that Wac1 p is required to achieve this checkpoint function through a transcriptional regulation of CLB2. Furthermore, we revealed that WACI was identical with ARP1 (actin-related protein). Arp1p, Nip100p and Jnm1p, which are components of the dynactin complex, are required to achieve the G2 arrest while keeping cells highly viable. These results indicate that the dynactin complex have a regulatory role in the novel checkpoint to monitor cell wall synthesis.

细胞增殖期间细胞周期事件的协调部分地通过监视系统来实现。芽殖酵母Saccharomyces cerevisiae的细胞周期进程受到限制性调控，细胞周期中的一些事件受到检测点的调控，这些检测点监测DNA损伤、DNA复制、纺锤体的完整性、纺锤体定位和细胞形态发生。目前还不清楚是否所有的检查点控制已经确定。我们发现，一个新的检查点存在耦合细胞壁重塑与纺锤体形成。1，3-β-葡聚糖合酶活性缺陷的FKSL突变体不能形成成熟芽。令人惊讶的是，这些细胞被复制后DNA捕获，但C1 b2 p水平很低，并且没有形成双极纺锤体。我们报告说，瓦迪（壁检查点缺陷）突变，取消了这种逮捕造成的积累C1 b2 p和CLB 2 mRNA，并导致形成双极纺锤体，尽管葡聚糖合成扰动。这些结果表明存在一种新的检查点机制来协调进入有丝分裂，并表明Wac 1 p是通过CLB 2的转录调节来实现该检查点功能所必需的。此外，我们发现WACI与ARP 1（肌动蛋白相关蛋白）是相同的。Arp 1 p、Nip 100 p和Jnm 1 p是dynactin复合物的组成部分，它们是实现G2期阻滞同时保持细胞高度活力所必需的。这些结果表明，dynactin复合物在新的检查点中具有调节作用，以监测细胞壁的合成。

项目成果

期刊论文数量（70）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break repair site initiated by meiosis-specific endonuclease VDE(PI-ScaI).

由减数分裂特异性核酸内切酶 VDE(PI-ScaI) 启动将 RecA 同源物 Dmc1p 和 Rad51p 招募到双链断裂修复位点。

DOI：
发表时间：
2005
期刊：
Mol Genet Genomics 275
影响因子：
0
作者：
Fukuda;T.
通讯作者：
T.

Piperazine propanol derivative as a novel antifungal targeting 1,3-beta-D-glucan synthase.

DOI：
10.1248/bpb.28.2138
发表时间：
2005-11
期刊：
Biological & pharmaceutical bulletin
影响因子：
2
作者：
O. Kondoh;Yukiko Inagaki;H. Fukuda;E. Mizuguchi;Y. Ohya;M. Arisawa;N. Shimma;Y. Aoki;M. Sakaitani;Takahide Watanabe
通讯作者：
O. Kondoh;Yukiko Inagaki;H. Fukuda;E. Mizuguchi;Y. Ohya;M. Arisawa;N. Shimma;Y. Aoki;M. Sakaitani;Takahide Watanabe

Analysis of the molecular interaction of the famesyl moiety of transducin through use of a photoreactive famesyl analog.

通过使用光反应性法呢基类似物分析转导蛋白法呢基部分的分子相互作用。