Development of a genome-scale in vitro transcription and translation-coupled system

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Transcription (RNA synthesis from DNA) and translation (Protein synthesis from RNA) are ubiquitous systems in all living organisms. An in vitro transcription system and an in vitro translation system were developed using stable cell components of a hyperthermophilic archaeon,Thermococcus kodakaraensis. A system for in vitro transcription and translation-coupled reactions was also constructed which enabled in vitro protein production from a DNA template. Furthermore, production of proteins from an operon, a cluster of genes transcribed as a single transcriptional unit, was also achieved.