Fine bubbles for biocatalytic processes: Microscale phenomena and novel applications

用于生物催化过程的细小气泡：微尺度现象和新颖应用

• 批准号: 501131738
• 负责人: Professor Dr. Andreas Liese
• 金额: --
• 依托单位: Institut für Technische Biokatalyse V-6
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/501131738?language=en
• 关键词: Fine; bubbles; biocatalytic; processes; Microscale

Biotransformations, where one of the reactants is supplied via the gas phase, especially in the case of oxidations, are still a challenge. The aeration of enzymatic reactions using fine bubbles, whose diameters are less than 100 µm, has been investigated by focusing on hydrodynamics and mass transfer effects as well as reaction kinetics of enzymatic systems. However, the fundamental scientific questions regarding the local processes taking place at the gas-liquid interface of a dissolving fine bubble are still to be targeted. This is even more important, if the interaction between a fine bubble and an immobilized enzyme on a surface is addressed, especially, on the background of increasing attention to structured packings as carrier materials for enzymes. The local mass transfer rate and oxygen concentration gradient at the interface is expected to be strongly affected by the Laplace pressure with decreasing bubble diameter. For mass transfer limited enzymatic reactions this mass transfer enhancement and the interaction between the enzymes and the gas-liquid interface are central aspects influencing the effectivity of the reaction. Using Laser Induced Fluorescence (LIF) measurements local mass transfer phenomena can be studied. With the help of these measurements the effect that enzymes are more stable during fine bubble aeration compared to conventional aeration, as demonstrated in the predecessor project, will be analyzed. In this context, investigation of the concentration boundary layer conditions on enzyme denaturation will be carried out for a better understanding of the acting mechanisms.

生物转化，其中一种反应物是通过气相提供的，特别是在氧化的情况下，仍然是一个挑战。通过关注流体力学和传质效应以及酶系统的反应动力学，研究了使用直径小于100 µm的细气泡进行酶反应的通气。然而，关于溶解细气泡的气-液界面处发生的局部过程的基本科学问题仍有待于针对。如果解决了表面上的细气泡和固定化酶之间的相互作用，特别是在越来越关注规整填料作为酶的载体材料的背景下，这甚至更重要。局部传质速率和氧气浓度梯度在界面处预计将强烈影响的拉普拉斯压力与气泡直径减小。对于传质受限的酶促反应来说，这种传质增强以及酶与气液界面之间的相互作用是影响反应有效性的核心方面。使用激光诱导荧光（LIF）测量可以研究局部传质现象。在这些测量的帮助下，将分析与传统曝气相比，细气泡曝气过程中酶更稳定的效果，如前一个项目所示。在这种情况下，研究浓度边界层条件对酶变性的影响，以更好地理解其作用机制。