3D x-ray microscope

现代生物医学研究需要对组织环境中的微观解剖或组织病理学结构进行三维 (3D) 高分辨率可视化。汉诺威医学院 (MHH) 可用的光学显微镜方法与电子显微镜和 X 射线结构分析方法之间，在分辨率和最大可表示体积方面存在差距。由于 MHH 和相关机构非常需要众多工作组来回答相关问题，因此我们正在申请 3D X 射线显微镜，旨在填补这一空白。在动物实验中，这使得能够以高分辨率对整个器官（包括肾脏、肺或大脑）进行 3D 分析，从而清晰地记录微观细节。该设备对于成功实施当前和计划中的部分第三方资助的项目和研究协会（包括德国肺研究中心）具有极其重要的意义，并将被集成到 MHH 的电子显微镜核心设施中。因此，它是对核心设施中可用的 3D 电子显微镜设备（例如聚焦离子束和串行块面扫描电子显微镜）的极好补充，可实现超微结构水平的非常高效的多模态相关 3D 成像。电子显微镜核心设施和解剖研究所的员工保证提供有力的支持。经过指导后，所有感兴趣的工作组都将获得访问权限。计划进行使用操作。因此，接受指导的科学家可以通过核心设施的预约系统进行预约，并独立生成X射线显微断层扫描数据集并使用提供的软件对其进行评估。

Modern biomedical research requires three-dimensional (3D), high resolution visualization of microscopic-anatomical or histopathological structures in the tissue context. Between the light microscopic methods available for this purpose at Hannover MedicalSchool (MHH) on the one hand and electron microscopic and X-ray structure analysis methods on the other hand, there is a gap in terms of resolution and the maximum representable volume. Since there is a considerable need for numerous working groups at MHH and associated institutions to answer relevant questions, we are applying for a 3D X-ray microscope with the aim of filling this gap. In the context of animal experiments, this allows a 3D analysis of entire organs (including kidneys, lungs or brain) to be carried out in high resolution and thus microscopic details to be clearly recorded. The device is of outstanding importance for the successful implementation of current and planned, partly third-party funded projects and research associations (including German Centre for Lung Research) and is to be integrated into the core facility for electron microscopy at MHH. It thus represents an excellent complementation to the 3D electron microscopic devices available at the core facility such as Focus-Ion-Beam and Serial-Block-Face Scanning Electron Microscopy to allows very efficient multimodal correlative 3D imaging down to the ultrastructural level. Employees of the core facility for electron microscopy and the Institute for Anatomy guarantee competent support. After instruction, all interested working groups will be given access. A usage operation is planned. Accordingly the instructed scientists can book appointments via the booking system of the core facility and independently generate X-ray microtomographic data sets and evaluate them with the software provided.