3D x-ray microscope

3D X 射线显微镜

• 批准号: 501811684
• 金额: --
• 依托单位: Medizinische Hochschule Hannover (MHH)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/501811684?language=en
• 关键词: 3D; ray; microscope

Modern biomedical research requires three-dimensional (3D), high resolution visualization of microscopic-anatomical or histopathological structures in the tissue context. Between the light microscopic methods available for this purpose at Hannover MedicalSchool (MHH) on the one hand and electron microscopic and X-ray structure analysis methods on the other hand, there is a gap in terms of resolution and the maximum representable volume. Since there is a considerable need for numerous working groups at MHH and associated institutions to answer relevant questions, we are applying for a 3D X-ray microscope with the aim of filling this gap. In the context of animal experiments, this allows a 3D analysis of entire organs (including kidneys, lungs or brain) to be carried out in high resolution and thus microscopic details to be clearly recorded. The device is of outstanding importance for the successful implementation of current and planned, partly third-party funded projects and research associations (including German Centre for Lung Research) and is to be integrated into the core facility for electron microscopy at MHH. It thus represents an excellent complementation to the 3D electron microscopic devices available at the core facility such as Focus-Ion-Beam and Serial-Block-Face Scanning Electron Microscopy to allows very efficient multimodal correlative 3D imaging down to the ultrastructural level. Employees of the core facility for electron microscopy and the Institute for Anatomy guarantee competent support. After instruction, all interested working groups will be given access. A usage operation is planned. Accordingly the instructed scientists can book appointments via the booking system of the core facility and independently generate X-ray microtomographic data sets and evaluate them with the software provided.

现代生物医学研究需要三维（3D），在组织背景下的显微解剖或组织病理学结构的高分辨率可视化。汉诺威医学院（MHH）用于此目的的光学显微镜方法与电子显微镜和x射线结构分析方法之间，在分辨率和最大可代表体积方面存在差距。由于MHH和相关机构的众多工作组需要回答相关问题，我们正在申请3D x射线显微镜，以填补这一空白。在动物实验中，这允许对整个器官（包括肾脏、肺或脑）进行高分辨率的3D分析，从而清晰地记录微观细节。该设备对于成功实施当前和计划中的，部分由第三方资助的项目和研究协会（包括德国肺研究中心）非常重要，并且将被整合到MHH的电子显微镜核心设施中。因此，它是对核心设施中可用的3D电子显微镜设备（如聚焦离子束和串行块面扫描电子显微镜）的极好补充，可以非常高效地进行多模态相关3D成像，直到超微结构水平。电子显微镜核心设备和解剖研究所的员工保证提供有力的支持。经指示后，所有感兴趣的工作组均可进入。已规划使用操作。因此，受指导的科学家可以通过核心设施的预约系统预约，并独立生成x射线显微层析成像数据集，并使用提供的软件进行评估。