3D X-RAY PHASE CONTRAST MICROSCOPE FOR SUBMICRON QUANTITATIVE BIOLOGICAL IMAGING

用于亚微米定量生物成像的 3D X 射线相衬显微镜

• 批准号: 9075769
• 负责人: MATTHEW J SILVA
• 金额: $ 101.59万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075769
• 关键词: Adipocytes; Adipose tissue; Animal Model; Arthritis; Biological; Biomedical Research; Cardiac development; Cardiovascular Diseases; Center Core Grants; Communities; Consultations; Direct Costs; Fracture Healing; Goals; Health; Image; Institutes; Institution; Joints; Malignant Neoplasms; Microscope; Minor; Mission; Missouri; Mus; Musculoskeletal; National Heart, Lung, and Blood Institute; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of General Medical Sciences; Neoplasm Metastasis; Osteocytes; Osteoporosis; Outcome; Phase; Porosity; Research; Research Infrastructure; Research Project Grants; Resolution; Roentgen Rays; Sampling; Services; Skeletal Development; Structure; System; Thick; Tissues; United States National Institutes of Health; Universities; Washington; X-Ray Computed Tomography; Zebrafish; articular cartilage; bone; imaging system; instrument; lung development; microscopic imaging; preclinical study; programs; ranpirnase; scoliosis; submicron

 DESCRIPTION (provided by applicant): We propose to purchase a ZEISS Xradia 520 Versa 3D X-ray microscope (XRM). This is a state-of-the-art imaging system for non-destructive, submicron resolution, high-contrast, 3D X- ray computed tomography imaging of biological tissues. There is no comparable system at our institution (Washington University) nor in our state (Missouri). We have assembled a group of 19 Major and Minor Users at our institution whose research is currently limited by lack of an instrument with the features of the Xradia 520 Versa. Their research programs will benefit greatly from local access to this instrument. These users are supported by 44 current NIH research grants from six institutes (NCI, NHLBI, NIA, NIAMS, NICDH, NIGMS), totaling $9.6 million direct costs/year. The instrument will be located in the Washington University Musculoskeletal Research Center, and managed through the existing Musculoskeletal Structure and Strength Core (MSSC; MJ Silva, Director). The MSSC is a service and consultation core supported by an NIH P30 Core Center grant (AR057235). Addition of the proposed instrument will enhance the technical capabilities of the core and provide access to state-of-the-art XRM imaging to the biomedical research community at Washington University. The research projects of the Major and Minor Users are focused on basic and pre-clinical studies related to many health conditions, including osteoporosis, arthritis cancer, cardiovascular disease, scoliosis and fracture healing. A common need is for high-resolution, high-contrast, non-destructive imaging of model organisms (e.g., mouse, zebrafish), which the proposed instrument will deliver. Imaging applications of the instrument include: bone porosity; vascularity in fracture healing and metastatic tumors; articular cartilage and joint tissues; bone microdamage; adipose tissue; skeletal, cardiac and lung development. The instrument will allow the users to assess outcomes that currently we cannot assess (e.g., osteocyte lacunar number, articular cartilage thickness, microvascular vessel volume, adipocyte volume) and to do so with non-biased sampling of 3D volumes. In closing, the proposed instrument will enhance the biomedical research infrastructure at Washington University, provide access to state-of-the-art imaging not available on our campus, and accelerate the pace of health-related research projects. This is consistent with the long-term goals and mission of Washington University.

 描述(由申请人提供)：我们建议购买ZEISS XRadia 520 Versa 3D X射线显微镜(XRM)。这是一种最先进的成像系统，用于对生物组织进行无损、亚微米分辨率、高对比度、3D X射线计算机断层成像。我们的机构(华盛顿大学)和我们州(密苏里州)都没有类似的制度。我们在我们机构组建了一个由19名主要和次要用户组成的小组，他们的研究目前由于缺乏具有XRadia 520功能的仪器而受到限制。他们的研究项目将极大地受益于当地对该仪器的使用。这些用户得到了来自六个研究所(NCI、NHLBI、NIA、NIAMS、NICDh、NIGMS)的44项当前NIH研究拨款的支持，每年的直接费用总额为960万美元。该仪器将设在华盛顿大学肌肉骨骼研究中心，通过现有的肌肉骨骼结构和力量核心(MSSC；MJ席尔瓦主任)进行管理。MSSC是由NIH P30核心中心拨款(AR057235)支持的服务和咨询核心。拟议中的仪器的加入将增强核心的技术能力，并向华盛顿大学的生物医学研究界提供获得最先进的XRM成像的途径。主要用户和次要用户的研究项目侧重于与许多健康状况相关的基础和临床前研究，包括骨质疏松症、关节炎、癌症、心血管疾病、脊柱侧弯和骨折愈合。一种常见的需求是高分辨率、高对比度、非破坏性的模式生物成像(例如，小鼠、斑马鱼)，这是拟议的仪器将提供的。该仪器的成像应用包括：骨孔率；骨折愈合和转移肿瘤中的血管；关节软骨和关节组织；骨微损伤；脂肪组织；骨骼、心脏和肺的发育。该仪器将允许用户评估目前我们无法评估的结果(例如，骨细胞陷窝数量、关节软骨厚度、微血管体积、脂肪细胞体积)，并使用3D体积的无偏采样进行评估。最后，拟议的仪器将增强华盛顿大学的生物医学研究基础设施，提供我们校园中无法获得的最先进的成像技术，并加快与健康相关的研究项目的步伐。这与华盛顿大学的长期目标和使命是一致的。