A systems biotechnology approach for improving substrate uptake during succinic acid production in a two-phase cultivation process

一种系统生物技术方法，用于改善两阶段培养过程中琥珀酸生产过程中的底物吸收

• 批准号: 501999334
• 负责人: Dr. Katja Bettenbrock
• 金额: --
• 依托单位: Max-Planck-Institut für Dynamik komplexer technischer Systeme
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/501999334?language=en
• 关键词: systems; biotechnology; approach; improving; substrate

Although the biotechnological production of succinic acid (succinate) with bacteria has already been very well-investigated, high productivity has hardly been achieved in the bioreactor. An aspect that has not yet been investigated, is the uptake of substrates such as glucose in the bacterial cells. In preliminary investigations, we have already examined some strain variants with modified uptake systems, but data could not be collected systematically. In conjunction with a theoretical approach in which a dynamic, mathematical model for the anaerobic growth and production behaviour is to be developed, the focus of the planned project is on improved substrate uptake and improved production of succinic acid. Essential points of the work program consist of the development and experimental testing of new strain variants, the integration of measured data into an ensemble of models and the design and optimization of a two-stage process for the production of succinic acid in the bioreactor.

虽然用细菌生产琥珀酸的生物技术已经得到了很好的研究，但在生物反应器中几乎没有实现高生产率。尚未研究的一个方面是细菌细胞中底物如葡萄糖的摄取。在初步研究中，我们已经检查了一些具有修饰的摄取系统的菌株变体，但无法系统地收集数据。结合开发厌氧生长和生产行为的动态数学模型的理论方法，计划项目的重点是改善底物吸收和提高琥珀酸生产。工作计划的要点包括新菌株变体的开发和实验测试，将测量数据整合到模型集合中，以及在生物反应器中生产琥珀酸的两阶段工艺的设计和优化。