A systems biotechnology approach for improving substrate uptake during succinic acid production in a two-phase cultivation process

虽然用细菌生产琥珀酸（琥珀酸）的生物技术已经得到了很好的研究，但在生物反应器中几乎没有实现高生产率。一个尚未被研究的方面是细菌细胞对底物如葡萄糖的摄取。在初步调查中，我们已经用改良的摄取系统检查了一些菌株变异，但无法系统地收集数据。结合一种理论方法，其中将开发厌氧生长和生产行为的动态数学模型，计划项目的重点是改善底物的吸收和改善琥珀酸的生产。工作计划的要点包括新菌株变体的开发和实验测试，将测量数据整合到模型集合中，以及在生物反应器中生产琥珀酸的两阶段工艺的设计和优化。

Although the biotechnological production of succinic acid (succinate) with bacteria has already been very well-investigated, high productivity has hardly been achieved in the bioreactor. An aspect that has not yet been investigated, is the uptake of substrates such as glucose in the bacterial cells. In preliminary investigations, we have already examined some strain variants with modified uptake systems, but data could not be collected systematically. In conjunction with a theoretical approach in which a dynamic, mathematical model for the anaerobic growth and production behaviour is to be developed, the focus of the planned project is on improved substrate uptake and improved production of succinic acid. Essential points of the work program consist of the development and experimental testing of new strain variants, the integration of measured data into an ensemble of models and the design and optimization of a two-stage process for the production of succinic acid in the bioreactor.