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放線菌の線状ゲノムのダイナミックな構造変化の解析

放线菌线性基因组动态结构变化分析

基本信息

批准号：
15013242
负责人：
木梨陽康
金额：
$ 2.75万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2003
资助国家：
日本
起止时间：
2003 至无数据
项目状态：
已结题

项目摘要

(1)Streptomyces griseusの線状染色体のダイナミックな構造変化の解析今年はこれまでの成果の多くを報文としてまとめることができた。すなわちMM9株においては、線状染色体の左右のアーム置換によって異常に長い末端逆位配列(terminal inverted repeats, TIRs)が形成されたことをJ.Bacteriol.に発表した。また301-22株においては、こうしてできたlong TIRsが更に欠失し、欠失末端同士がTIR内で結合して巨大パリンドローム構造(closed racket frame structure)が形成されたことを同じくJ.Bacteriol.(印刷中)に発表した。しかし、パリンドロームの先端部のクローン化が困難であることから、その塩基配列はまだ決定できていない。402-2株の染色体逆位については、2つの逆位部位及びそれに対応する親株DNAの詳細な比較によって、逆位に比較的大きなトランスポゾン様配列(約7kb)が関与していることが明らかになった。親株ではこの配列が1コピーあるのに対して、402-2株では2コピーある。それゆえ、402-2株では2コピー目のトランスポゾンが染色体に逆向きに挿入され、初めのコピーと相同組み換えを起こして2Mbにも及ぶ染色体逆位が起こったことが推定された。(2)S.coelicolor A3(2)の線状染色体とSCP1の相互作用の解析2106株においては、線状染色体と線状プラスミドSCP1が一点交差して2つのキメラ染色体が形成されたことを報文にまとめた(投稿中)。S.coelicolor A3(2)染色体と線状プラスミドSCP1の全塩基配列が明らかになったので、それを利用してJCM4979株のSCP1プラスミドラダーの構造をほぼ推定することができた。これをhybridizationとsdquencingによって更に確認する予定である。SCP1の末端蛋白ついては、その単離とTOF-MSによる解析を試みたが、まだ同定するには至っていない。

(1) the analysis of the results of this year's Streptomyces griseus-like chromosomes. In this study, the MM9 plant was divided into two groups: the left and right sides of the shape chromosome, the normal terminal reverse alignment (terminal inverted repeats, TIRs), the formation of the long terminal reverse alignment (J.Bacteriol). Please tell me the table. The results show that there are more defects in the long TIRs than in the control group. In combination with the same TIR at the end, there is a great deal of trouble in the construction of the J.Bacteriol (closed racket frame structure). (in printing) the table is not available. This is the first time to make a decision on how to solve the problem at the end. The chromosomal retrograde position of 402-2 strains is different from that of the other two strains, and the reverse position of the chromosome is different from that of the DNA plant. The reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the reverse position of the chromosome is different from that of the other two strains, the Each plant was divided into two groups: 1, 402-2, and 4-2. The chromosomes were inversely linked to the chromosomes, and the chromosomes were detected in the same tissue in the first trimester. The chromosomes were inversely located and the chromosomes were inversely located. The chromosomes were presumed to be negative. (2) S.coelicolor A3 (2) S.coelicolor A3 (2) "SCP1" interaction "analysis of 2106 plants", "chromosome", "chromosome", "SCP1", "one point difference", "two chromosomes", "chromosome" formation, "report", "report" (in the contribution). The complete sequence of S.coelicolor A3 (2) chromosomes was sequenced to show that the whole genome of JCM4979 was constructed by using SCP1 and DNA sequences of JCM4979 plants. Please hybridization the sdquencing to confirm that you are predetermined to receive more information. The SCP1 terminal protein was isolated from the TOF-MS to analyze the terminal protein from the terminal protein to the terminal protein.