NanoVidere - Photochromic Layers for Nanoscopy

NanoVidere - 用于纳米显微镜的光致变色层

• 批准号: 504588119
• 负责人: Professor Dr. Andreas Schmidt
• 金额: --
• 依托单位: Institut für Physikalische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/504588119?language=en
• 关键词: NanoVidere; Photochromic; Layers; Nanoscopy

This project aims to develop a new microscopy technique basing on high-resolution far field fluorescence microscopy such as the stimulated emission depletion microscopy (STED) to optically examine surfaces with nanoscale resolution. To improve the resolution the surfaces will be covered by photochromic layers (absorbance modulation layers; AML) which reversibly change their color by photoirradiation by an activating laser to decrease the transmittance. By means of a doughnut mode of the activating laser, an aperture is formed for the measuring laser, which can solely detect signals through the transparent hole in the middle of the doughnut. The AML group of the project (Institutes of Organic Chemistry and Physical Chemistry as well the Max-Planck Institute for Biophysical Chemistry, Department NanoBiophotonik) will prepare layers containing 1,2-bis(thienyl)ethenes as photochromic molecules in a polymeric or an organic matrix. The objective of this subproject is the identification of suitable photochromic molecules and their synthesis for further investigations, as no photochromic molecule reported so far fulfills the specific requirements for application in nanoscopy. The suitability to prepare absorbance modulation layers will i.a. be determined by the fatigue resistencies, irradiation wavelengths, quantum efficiencies for the reversible switching processes, transparencies, and resistance towards crystallization in the matrix. After the syntheses, characterizations, and evaluations in collaboration with our project partners, suitable photochromes will be delivered for application in nanoscopy.

该项目旨在开发一种基于高分辨率远场荧光显微镜（如受激发射耗尽显微镜（STED））的新显微镜技术，以纳米级分辨率对表面进行光学检查。为了提高分辨率，表面将被光致变色层（吸光度调制层; AML）覆盖，该光致变色层通过活化激光的光照射可逆地改变其颜色以降低透射率。通过激活激光器的环形模式，形成用于测量激光器的孔径，其可以仅通过环形中间的透明孔来检测信号。该项目的AML小组（有机化学和物理化学研究所以及马克斯-普朗克生物物理化学研究所，NanoBiophotonik部门）将在聚合物或有机基质中制备含有1，2-双（噻吩基）乙烯作为光致变色分子的层。这个子项目的目的是确定合适的光致变色分子和它们的合成进行进一步的研究，因为迄今为止还没有报道的光致变色分子满足纳米显微镜应用的特定要求。制备吸光度调制层的适用性将由抗疲劳性、辐射波长、可逆转换过程的量子效率、结晶性和对基质中结晶的抵抗力来确定。在与我们的项目合作伙伴合作进行合成、表征和评估后，将提供合适的光致变色材料用于纳米显微镜。