Tomographic X-Ray Microscope System

X射线断层显微镜系统

• 批准号: 8246978
• 负责人: Simon R Cherry
• 金额: $ 59.37万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-15 至 2013-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246978
• 关键词: 3-Dimensional; Animal Disease Models; Animal Model; Area; Arterial Fatty Streak; Atherosclerosis; Biocompatible Materials; Biological; Biopsy Specimen; Cell Culture Techniques; Cellularity; Complex; Core Facility; Data Set; Disease; Engineering; Funding; Genomics; Goals; Gold; Image; Imaging Techniques; Implant; Joints; Malignant Neoplasms; Mechanics; Methods; Microscope; Molecular; Morphology; Musculoskeletal Diseases; Names; Operative Surgical Procedures; Optics; Organ; Pathology; Patients; Phase; Property; Public Health; Regenerative Medicine; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Roentgen Rays; Secure; Source; Specimen; Staining method; Stains; Structure; System; Techniques; Technology; Therapeutic; Therapy Evaluation; Tissues; United States National Institutes of Health; X-Ray Tomography; bone imaging; calcification; density; detector; improved; in vivo; light scattering; nanodiagnostic; nanoparticle; nanorod; nanotherapeutic; reconstruction; regenerative therapy; scaffold; sensor; tumor

DESCRIPTION (provided by applicant): The goal of this proposal is to secure funding to add an X-ray "microscope" to the Center for Molecular and Genomic Imaging, a core facility at UC Davis that provides centralized infrastructure and expertise to conduct imaging studies in animal models and biospecimens. We propose to purchase an Xradia microXCT-200 high- resolution X-ray tomography microscope system that includes a 90kV microfocus X-ray source, a 4 M pixel CCD sensor, selectable detector optics with magnifications ranging from 4X to 40X, and a workstation for reconstruction and display of the large CT datasets. The system will be used for high resolution imaging of a range of biospecimens from 3-D cell culture, animal model tissues and patient biopsy samples in applications that span imaging of calcifications in tumors and atherosclerotic plaques, imaging of bone and joint pathologies and regenerative therapies, evaluation of the 3-D structure and mechanical properties of biomaterials and scaffolds, and imaging of the distribution of high-density (e.g. gold) nanoparticles and nanorods, to name just a few. The proposed system can achieve a spatial resolution (10% MTF) of < 1 ?m using the 40X optics and can image specimens up to 50 mm in size (20 mm with 40X detector). The system will be placed in our core facility and used to support and aid NIH-funded research in diverse areas such as musculoskeletal disease, atherosclerosis, cancer, regenerative medicine, biomaterials, nanodiagnostics and nanotherapeutics. The system will directly support improved understanding of disease through high-resolution, high contrast imaging of surgical or biopsy specimens from animal models of patients, the characterization of engineered implants, constructs and tissues, and the effects of a range of therapeutic strategies. PUBLIC HEALTH RELEVANECE: Imaging is a powerful technology for visualizing complex 3-D biological tissues and changes in these tissues that occur through disease. The proposed X-ray tomography microscope system utilizes advanced optics and phase contrast methods to achieve unprecedented resolution (~ 1 ¿m) and high contrast images across large volumes of biological tissue without staining or sectioning, providing undistorted and highly quantitative images of tissue morphology. This allows entire biopsy specimens, or organs/tissues from animal models to be imaged, and a wide range of morphological parameters (e.g. cellularity, density, vascularity, microarchitecture) to be assessed across the entire specimen, something that cannot be done with optical techniques due to the high degree of light scatter within biological tissues. The proposed X-ray microscope will be integrated in our imaging center with existing 2D and 3D in vivo imaging systems to provide a comprehensive resource for NIH funded researchers to study animal models of disease using imaging techniques.

描述（由申请人提供）：该提案的目标是获得资金，为分子和基因组成像中心添加一台 X 射线“显微镜”，该中心是加州大学戴维斯分校的核心设施，提供集中的基础设施和专业知识，以在动物模型和生物样本中进行成像研究。我们建议购买 Xradia microXCT-200 高分辨率 X 射线断层扫描显微镜系统，该系统包括 90kV 微焦点 X 射线源、4 M 像素 CCD 传感器、放大倍数范围为 4 倍至 40 倍的可选探测器光学器件，以及用于重建和显示大型 CT 数据集的工作站。该系统将用于对来自 3D 细胞培养物、动物模型组织和患者活检样本的一系列生物样本进行高分辨率成像，其应用涵盖肿瘤和动脉粥样硬化斑块中的钙化成像、骨和关节病理和再生治疗的成像、生物材料和支架的 3D 结构和机械性能的评估以及高密度分布的成像（例如，生物材料）。 金）纳米粒子和纳米棒，仅举几例。所提出的系统可以使用 40X 光学器件实现 < 1 µm 的空间分辨率（10% MTF），并且可以对尺寸最大为 50 毫米的样本进行成像（使用 40X 探测器时为 20 毫米）。该系统将放置在我们的核心设施中，用于支持和帮助 NIH 资助的多个领域的研究，例如肌肉骨骼疾病、动脉粥样硬化、癌症、再生医学、生物材料、纳米诊断和纳米治疗。该系统将通过对患者动物模型的手术或活检标本进行高分辨率、高对比度成像、工程植入物、结构和组织的表征以及一系列治疗策略的效果，直接支持提高对疾病的了解。 公共卫生相关性：成像是一种强大的技术，用于可视化复杂的 3D 生物组织以及这些组织因疾病而发生的变化。所提出的 X 射线断层扫描显微镜系统利用先进的光学和相衬方法，无需染色或切片即可在大量生物组织中实现前所未有的分辨率（约 1 µm）和高对比度图像，从而提供组织形态的不失真且高度定量的图像。这样可以对整个活检标本或动物模型的器官/组织进行成像，并评估整个标本的各种形态参数（例如细胞结构、密度、血管分布、微结构），这是光学技术无法完成的，因为生物组织内的光散射程度很高。拟议的 X 射线显微镜将在我们的成像中心与现有的 2D 和 3D 体内成像系统集成，为 NIH 资助的研究人员使用成像技术研究疾病动物模型提供全面的资源。