Studies on enzymes for organic sulfur compounds-cleavage and formation of carbon-sulfur bond

研究了二苯并噻吩（DBT）和岩藻聚糖硫酸酯的酶作用，DBT存在于石油中，岩藻聚糖硫酸酯存在于藻类中。由于石油燃烧会释放出酸雨物质，因此在燃烧前必须进行“脱硫”。DBT降解酶适用于生物脱硫，本研究采用基因工程的方法对DBT降解酶进行改良。褐藻糖胶是一种具有抗癌等生理活性的高分子化合物，降低褐藻糖胶的分子量有助于发现新的活性物质。本研究分离了新型岩藻聚糖降解菌，并对其酶活性进行了检测。

Enzymes acting on dibenzothiophene (DBT) and fucoidan were investigated ; DBT was contained in petroleum and fucoidan in algae. Since combustion of petroleum released substances for acid rain, "desulfurization" is a necessary step before combustion. DBT degrading enzymes was applicable to biodesulfurization, and the enzyme was improved by genetic engineering method in this study. Fucoidan is a high-molecular-weight compound with physiological activities such as anticancer one, and decreasing the molecular weight would lead to finding of new activities. Novel fucoidan-degrading microorganisms were isolated, and enzyme activities were detected in this study.