The effect of cancer-targeted liposome with viral proteins on staining and visualizing cancer cells

我们开发了一种新型荧光标记的癌症靶向脂质体，该脂质体表面偶联sindbis病毒蛋白（SIN杂交脂质体），可用于肿瘤成像，并优化了生物分布分析和术中导航系统。将聚乙二醇化脂质体与紫外线灭活的Sindbis病毒蛋白通过超离心纯化，制备了Sindbis杂交脂质体。用透射电镜观察了SIN杂化脂质体的结构。将癌细胞与包封FITC-葡聚糖的SIN杂交或非SIN杂交罗丹明标记脂质体一起培养，共形显微镜和流式细胞术显示，与非SIN杂交脂质体相比，SIN杂交脂质体处理的癌细胞在细胞膜上和细胞质中检测到大量的罗丹明荧光。此外，将每个脂质体全身注射到荷瘤小鼠模型中，并使用IVIIS lumina系统成像以确定其分布。SIN杂化脂质体优先在肿瘤部位分布和积聚，提高了信噪比。

We developed a novel fluorescence-labeled cancer-targeted liposome coupled to sindbis virus proteins (SIN hybrid liposome) on the surface that can be used for imaging tumors and optimized for a bio-distribution analysis and an intra-operative navigation system. SIN hybrid liposome was prepared by mixing the pegylated liposome and UV-inactivated Sindbis virus protein that were purified by an ultracentrifuge. The structure of SIN hybrid liposome was observed by a Transmission Electron Microscope. When cancer cells were incubated with SIN hybrid or non-SIN hybrid rhodamin labeled liposomes encapsulating FITC-dextran, both conforcal microscopy and flow cytometry revealed that a large number of rhodamin fluorescence on the cell membrane and FITC in the cytoplasm were detected in the cancer cells treated with SIN hybrid liposomes compared with non-SIN hybrid liposomes. Furthermore, each liposome was systemically injected in tumor bearing mouse model and imaged for distribution using IVIIS lumina systems. SIN hybrid liposome showed distribution and accumulation at the tumor site preferentially and improved the signal-to-noise ration.