Identification of proteins associated with oral cancer by global proteomic approach.

通过整体蛋白质组学方法鉴定与口腔癌相关的蛋白质。

• 批准号: 16209059
• 负责人: TANZAWA Hideki
• 金额: $ 31.95万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209059/
• 关键词: oral cancer; profiling of protein expression; 2D-DIGE; MALDI-TOF; MS; peptide mass fingerprinting; タンパク発現プロファイリング; _2D-DIGE; petide mass fingerprinting

Proteomic technologies provide excellent tools for rapid screening of a large number of potential biomarkers in malignant cells. To gain insight into the molecular mechanisms of carcinogenesis and to identify potential biomarkers for oral cancer, we performed proteomic profiling between oral cancer and normal oral mucosa using fluorescent two-dimensional difference in-gel electrophoresis. Proteins with a 【greater than or equal】2-fold change in expression were considered significant. The spots of interest were digested and identified by matrix-assisted laser desorption/ionization time-of-flight peptide mass finger-printing. These results of proteomic analysis were confirmed by Western blotting, immunohistochemical staining, microarray analysis, and real time RT-PCR method.Twenty-two proteins were identified as differentially expressed between oral cancer and normal oral mucosa. Of these, 9 spots were up-regulated and 13 were down-regulated in oral cancer compared to Normal oral mucosa. These spots included the cancer-related proteins ; annexin A1, heat shock protein 27, lamin A/C, interleukin 1 receptor antagonist, serine proteinase inhibitor clade B5, stathmin 1, superoxide dismutase 2. Especially, maspin and stathmin were suggested to be useful biomarkers for oncogenesis.

蛋白质组学技术为快速筛选恶性细胞中大量潜在的生物标志物提供了极好的工具。为了深入了解口腔癌发生的分子机制，并找出口腔癌潜在的生物标志物，我们利用荧光双向差示凝胶电泳法对口腔癌和正常口腔黏膜进行了蛋白质组学研究。表达变化[大于或等于]2倍的蛋白质被认为是显著的。利用基质辅助激光解吸/电离飞行时间肽质量指纹图谱对感兴趣的斑点进行消化和鉴定。蛋白质组分析结果通过Western blotting、免疫组织化学染色、微阵列分析和实时定量RT-PCR方法得到证实。其中，与正常口腔黏膜相比，口腔癌组织中有9个点表达上调，13个点表达下调。这些斑点包括：膜联蛋白A1、热休克蛋白27、层蛋白A/C、白介素1受体拮抗剂、丝氨酸蛋白酶抑制因子B5、Stathmin 1、超氧化物歧化酶2，特别是Maspin和stathmin是肿瘤发生的有用生物标志物。

项目成果

Ubiquitous mitochondrial creative kinase downregulated in oral squamous cell carcinoma

口腔鳞状细胞癌中普遍存在的线粒体创造性激酶下调

• 发表时间: 2006
• 期刊: Br J Cancer. 94
• 作者: Onda T;Uzawa K;Endo Y;Bukawa H;Yokoe H;Shibahara T;Tanzawa H
• 通讯作者: Tanzawa H

Ubiquitous mitochondrial creatine kinase downregulated in oral squamous cell carcinoma.

• DOI: 10.1038/sj.bjc.6602986
• 发表时间: 2006-03-13
• 期刊: British journal of cancer
• 影响因子: 8.8

Plasma membrane Ca^<2+> ATPase isoform 1 down-regulated in human oral cancer

质膜 Ca^<2 > ATPase 亚型 1 在人类口腔癌中下调

• 发表时间: 2006
• 期刊: Oncol Rep 15
• 作者: Saito K;Uzawa K;Endo Y;Kato Y;Nakashima D;Ogawara K;Shiba M;Bukawa H;Yokoe H;Tanzawa H
• 通讯作者: Tanzawa H

WISP-2 expression in human salivary gland tumors.

• DOI: 10.3892/ijmm.17.4.567
• 发表时间: 2006-04
• 期刊: International journal of molecular medicine
• 影响因子: 5.4
• 作者: Y. Kouzu;K. Uzawa;Masaki Kato;M. Higo;Y. Nimura;K. Harada;T. Numata;N. Seki;Mitsunobu Sato;H. Tanzawa

Overexpression of stathmin in oral squamous-cell carcinoma : correlation with tumor progression and pool prognosis.

口腔鳞状细胞癌中 Stathmin 的过度表达：与肿瘤进展和池预后的相关性。

• 发表时间: 2006
• 期刊: Br J Cancer. 94(5)
• 作者: Kouzu Y;Uzawa K;Koike H;Saito K;Nakashima D;Higo M;Endo Y;Kasamatsu A;Shiiba M;Bukawa H;Yokoe H;Tanzawa H.
• 通讯作者: Tanzawa H.