Development of dental implant with support of periodontal ligament

牙周膜支持牙种植体的研制

• 批准号: 10557159
• 负责人: TAKATA Takashi
• 金额: $ 8.06万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557159/
• 关键词: Dental Implant; Periodontal ligament; Cell transplantation; Collagen membrane; Growth factors; Hydroxyapatite; コラーゲン膜; 人工材料; コラーゲン; 培養; 再生医学; 組織工学

To develop a new dental implant system with the support of periodontal ligament (PDL), we studied(1) modification of biological characteristics of PDL cells in different condition in vitro,(2) proper vehicles of PDL cells for conjugation to a hydroxyapatite (HA) implant, and(3) histological structure of peri-implant tissue around PDL cell conjugated HA implants.The studies showed(1) PDL cells showed their phenotypic characteristics in vitro and the growth and differentiation were modified with growth factors including FGF, PDGF and EGF and mechanical stress.(2) For PDL cell conjugation and transplantation with HA implants, membranous collagen was the most suitable vehicle.(3) At 6 months after the implantation of PDL cell conjugated HA implants, PDL-like connective tissue was formed between the implant and bone, but no true cementum deposition with inserting collagen fibers was observed.Although, in the present studies, we could not observe true PDL support around implants by PDL cell conjugation, the results give useful information for the further improvement of the vehicle and conjugation methods for PDL cell transplantation with implants.

为了开发一种新型的具有牙周韧带（PDL）支持的种植体系统，我们研究了(1)不同条件下PDL细胞生物学特性的体外修饰，(2)PDL细胞与羟基磷灰石（HA）种植体结合的合适载体，以及(3)PDL细胞结合的HA种植体周围组织的组织学结构。研究表明：(1)PDL细胞在体外表现出表型特征，FGF、PDGF、EGF等生长因子和机械应力对其生长分化进行了修饰。(2)用透明质酸植入体结合和移植PDL细胞时，膜性胶原是最合适的载体。(3) PDL细胞结合HA种植体植入6个月后，种植体与骨之间形成PDL样结缔组织，但未见真正的骨质沉积并插入胶原纤维。虽然在目前的研究中，我们无法通过PDL细胞偶联观察到PDL在种植体周围的真实支持，但这一结果为进一步改进PDL细胞与种植体移植的载体和偶联方法提供了有用的信息。

项目成果

Matsuda, N.: "Role of epidermoid growth factor and its receptor in mechanical stress-induced differentiation of human periodontal ligament cells in vitro"Arch.Oral Biol.. 43. 987-997 (1998)

Matsuda, N.：“表皮样生长因子及其受体在体外机械应力诱导的人牙周膜细胞分化中的作用”Arch.Oral Biol.. 43. 987-997 (1998)

Matsuda, N., Morita, N., Matsuda, K., Watanabe, M.: "Proliferation and differentiation of human osteoblastic cells associated with differential activation of MAP kinases in response to epidermal growth factor, hypoxia, and mechanical stress in vitro"Bioch

Matsuda, N.、Morita, N.、Matsuda, K.、Watanabe, M.：“人成骨细胞的增殖和分化与 MAP 激酶响应表皮生长因子、缺氧和体外机械应力的差异激活相关”

Takata T.: "Attachment, proliferation and differentiation of periodontal ligament cells on various GTR membranes."J.Periodont Res.. 36(印刷中). (2001)

Takata T.：“牙周膜细胞在各种 GTR 膜上的附着、增殖和分化。”J.Periodont Res.. 36（印刷中）。

金田俊彦: "歯周靭帯細胞の増殖・分化に関する研究"日本歯周病学会会誌. 41. 113 (1999)

金田敏彦：“牙周膜细胞增殖和分化的研究”日本牙周病学会杂志41. 113（1999）。

T.TAKATA: "Migration of Osteoblastic Cells on Various GTR/GBR Membranes."J.Dent.Res.. 78. 189 (1999)

T.TAKATA：“成骨细胞在各种 GTR/GBR 膜上的迁移。”J.Dent.Res.. 78. 189 (1999)