Basic studies for the development of a new dental implant system with the support of periodontal ligament

本实验建立了牙周膜细胞的培养体系，并对其在不同培养条件下的形态和功能特性进行了研究，为牙周膜支持下新型种植体的研究奠定基础。此外，我们还观察了牙种植体材料羟基磷灰石(HA)上PDL细胞的体外行为。这些研究表明，培养的PDL细胞含有成牙骨质细胞/成骨细胞系细胞的细胞表型，这些细胞可以在HA上沉积牙骨质/骨样矿化组织。这些发现为进一步研究HA-PDL细胞复合体植入骨中提供了科学依据，HA-PDL细胞复合体比单独植入的HA表现出更少的骨僵硬程度。虽然在本研究的实验模型中，我们不能证明在HA上具有功能构筑的PDL，但这一发现似乎值得支持通过在种植体表面应用培养的PDL细胞来实现对种植体周围的PDL的支持的假说，并为牙种植学开辟新的前景奠定了基础。提示应进一步研究PDL细胞的增殖分化机制，改进培养的PDL细胞应用于种植材料的方法。

To establish the fundamental background for the development of a new dental implant system with the support of periodontal ligament (PDL), we established culture systems for PDL cells and characterized their morphological and functional properties in different culture conditions. We also observed behaviors of PDL cells on hydroxy appatite (HA), that is one of the representative dental implant material, in vitro. Those studies showed that cultured PDL cells contain a cellular phenotype of the cementoblast/osteoblast lineage cells, and these cells could deposit cementum/bone-like mineralized tissue on HA.These findings could give scientific base for a further study on the implantation of a HA-PDL cells complex into the bone, where the HA-PDL cells complex showed less degree of ankylosis of bone than HA implanted alone. Although we could not demonstrated PDL with functional architectures on HA in the experimental models used in the present studies, the findings seemed to be worthy to support the hypothesis that the PDL-support around a dental implant can be realized by applying cultured PDL cells on a surface of implant, and also gave the fundamentals to open a new prospectivc in dental implantology. It was strongly suggested that further studies on the mechanism of proliferation and differentiation of PDL cells and the improvement of the method for application of cultured PDL cells on implant materials.