Association analysis of the angiotensinogen gene polymorphism and life style with childhood essential hypertension.

我们检测血管紧张素原基因变异（AA，Aa，aa）在131名日本儿童使用聚合酶链反应。测量身高、体重、臂围、血压，并编制高血压生活方式和遗传因素调查表。结果表明：1）aa基因型频率为0.59，a等位基因频率为0.76。在这些日本儿童中，基因型频率与Hardy-Weinberg平衡没有显著差异。2）我们发现三种基因型之间没有显著关系（AA，Aa，aa）和收缩压或舒张压。3）比较aa基因型和AA + Aa基因型儿童的几个变量时，aa儿童的收缩压高于AA + Aa儿童，4）原发性高血压家族史、生活方式与儿童血压无相关性。5）多元回归分析表明，基因型是收缩压的独立影响因素，这些结果表明，血管紧张素原的这种分子变异体可能在收缩压升高中起一定作用。日本儿童的血压调节。

We detected the angiotensinogen gene variant (AA,Aa, aa) in 131 Japanese children using a polymerase chain reaction. We also measured height, weight, arm cicumference and blood pressure (BP), and set up a questionnaire concerning the life style and heredity for hypertension. Informed consent was obtained from parents to examine the blood chemistry and angiotensinogen gene variants.The following results were obtained.1) The genotype frequency of aa was 0.59, and the allele frequecy of a was 0.76. Genotype frequencies were not significantly different from Hardy-Weinberg's equilibrium in these Japanese children.2) We found no significant relationship between the three genotypes (AA,Aa, aa) and either systolic or diastolic BP.3) When comparing several variables in children with the aa genotype and the AA + Aa genotypes, systolic BP in children with aa was higher than in those with AA + Aa, although this was not statistically significant.4) No relationship was found between children's BP and the family history of essential hypertension or their life style.5) By multiple regression analysis, the genotype was a statistically significant independent factor of systolic BP.Thsese findings suggest that this molecular variant of the angiotensinogen may play some role in the regulation of BP in Japanese children.