Association analysis of the angiotensinogen gene polymorphism and life style with childhood essential hypertension.

我们用聚合酶链式反应检测了131名日本儿童的血管紧张素原基因变异(AA、AA、AA)。测量身高、体重、臂围和血压，并编制高血压生活方式和遗传情况调查问卷。经父母知情同意后进行血液生化和血管紧张素原基因变异检测，结果如下：1)AA基因频率为0.59，等位基因频率为0.76。这些日本儿童的基因频率与Hardy-Weinberg平衡值无显著差异。2)我们发现AA、AA、AA三种基因型与儿童的收缩压或舒张压均无显著关系。3)当比较AA基因和AA基因儿童的几个变量时，AA儿童的收缩压高于AA儿童，但差异无统计学意义。4)未发现儿童血压与高血压家族史或生活方式的关系。5)通过多元回归分析，该基因是影响收缩压的一个有统计学意义的独立因素。这些结果表明，血管紧张素原的这种分子变异可能在日本儿童的血压调节中起一定作用。

We detected the angiotensinogen gene variant (AA,Aa, aa) in 131 Japanese children using a polymerase chain reaction. We also measured height, weight, arm cicumference and blood pressure (BP), and set up a questionnaire concerning the life style and heredity for hypertension. Informed consent was obtained from parents to examine the blood chemistry and angiotensinogen gene variants.The following results were obtained.1) The genotype frequency of aa was 0.59, and the allele frequecy of a was 0.76. Genotype frequencies were not significantly different from Hardy-Weinberg's equilibrium in these Japanese children.2) We found no significant relationship between the three genotypes (AA,Aa, aa) and either systolic or diastolic BP.3) When comparing several variables in children with the aa genotype and the AA + Aa genotypes, systolic BP in children with aa was higher than in those with AA + Aa, although this was not statistically significant.4) No relationship was found between children's BP and the family history of essential hypertension or their life style.5) By multiple regression analysis, the genotype was a statistically significant independent factor of systolic BP.Thsese findings suggest that this molecular variant of the angiotensinogen may play some role in the regulation of BP in Japanese children.