Analysis of intercellular signaling molecules working in organ development and differentiation in plants

为了研究细胞间信号系统在器官形成和发育中的作用，我们采用了几种方法。我们在花椰菜头的外胞体提取物中发现了一个小的LTP蛋白，它在茎尖分生组织的形成和维持中起作用。遗传分析表明，三聚体g蛋白参与调节茎和根分生组织功能的肽激素受体下的胞内信号通路。我们发现了一组新的酪氨酸磺酸糖肽在植物发育过程中作为细胞间信号具有重要作用。我们研究了microRNA165/166在发育叶片中形成正面和背面边界的作用。此外，我们开发了在单个植物细胞中诱导特定基因或使用激光束使单个细胞失活的仪器系统

In order to examine intercellular signaling systems working in organ formation and development, we took several approaches. We identified small LTP proteins in apoplastic extracts of cauliflower heads having a role of formation and maintenance of shoot apical meristem. Genetic approach revealed that trimeric G-protein is involved in the intracellular signing pathway under the peptide hormone receptors that regulate shoot and root meristem function. We showed novel group of tyrosine-sulfated glycopeptides had important roles as intercellular signals in plant development. We investigated action of microRNA165/166 in forming the boundary between the adaxial- and the abaxial-regions in developing leaves. In addition, we developed instrumental systems inducing specific gene(s) in a single plant cell or inactivating a single cell by use of laser beams