Genetic Program Controlling Plant Organogenesis

本研究以拟南芥、水稻、牵牛花、百日菊、烟草等高等植物为研究对象，旨在阐明植物器官发生的遗传程序控制。（1）突变体的分离：影响花器官发生（Okada，后藤）、分生组织形成（町田，Tasaka）、叶发育（町田）、根生长（Okada）、根毛发育（Okada）、细胞分裂素非依赖性生长（Kakimoto）、温度敏感的芽和/或根再生（Sugiyama）和芽向重力性（Tasaka）的拟南芥突变体。转座子标记的矮秆和胎生突变体的水稻（Hirochika，Matsuoka）。(2)来自拟南芥突变体的基因克隆：HY 5（Okada）、CAPRICE（Okada）、CKI 1（Kakimoto）、杯形子叶2（Tasaka）、SPIRAL 1（Hashimoto）和JAM（町田）。(3)牵牛花突变基因的遗传分析(4)从叶肉细胞到导管分子的细胞分化的生化和分子分析。(5)花序特异性基因的筛选（Kohchi）。(6)在拟南芥（町田）或水稻（Hirochika）中产生标记系统。研究结果发表在国际科学期刊上，并在会议上作了报告。

This project aimed to clarify Genetic Program Controling Plant Organogenesis using higher plants such as Arabidopsis, rice, morning glory, Zinnia, and tobacco. Major results obtained in the year project are : (1) Isolation of mutants : Arabidopsis mutants affecting flower organogenesis (Okada, Goto), meristem formation (Machida, Tasaka), leaf development (Machida), root growth (Okada), root hair development (Okada), cytokinin-independent growth (Kakimoto), temperaturesensitive shoot and/or root regeneration (Sugiyama), and shoot gravitropism (Tasaka). Transposon-taggad dwarf, and viviporous mutants of rice (Hirochika, Matsuoka). (2) Gene cloning from Arabidopsis mutants : HY5 (Okada), CAPRICE (Okada), CKI1 (Kakimoto), CUP-SHAPED COTYLEDON2 (Tasaka), SPIRAL1 (Hashimoto), and JAM (Machida). (3) Genetic analysis of MUTABLE gene of morning glory (Iida). (4) Biochemical and Molecular analysis of cell differentiation from leaf mesophyll cells to tracheary elements (Fukuda). (5) Screening of inflorescence-specific genes (Kohchi). (6) Generation of tagging system in Arabidopsis (Machida) or in rice (Hirochika). The results were published in international scientific journals and reported in conferences.