Genetic Program Controlling Plant Organogenesis

控制植物器官发生的遗传程序

• 批准号: 06278103
• 负责人: OKADA Kiyotaka
• 金额: $ 163.52万
• 依托单位: Kyoto University (1995-1997)Okazaki National Research Institutes (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06278103/
• 关键词: Plant orgnanogenesis; Cell differentiation; Intercellular signaling; Arabidopsis; Tobacco; Rice; Mutant analysis; Transcription factor; 花芽形成; ホメオボックス遺伝子; 植物形態形成; 植物器官プラン; トランスジェニック植物; シグナル伝達; 転写因子; 植物器官; 形態形成; 遺伝的プログラム; 転写調節因子; 遺伝子導入

This project aimed to clarify Genetic Program Controling Plant Organogenesis using higher plants such as Arabidopsis, rice, morning glory, Zinnia, and tobacco. Major results obtained in the year project are : (1) Isolation of mutants : Arabidopsis mutants affecting flower organogenesis (Okada, Goto), meristem formation (Machida, Tasaka), leaf development (Machida), root growth (Okada), root hair development (Okada), cytokinin-independent growth (Kakimoto), temperaturesensitive shoot and/or root regeneration (Sugiyama), and shoot gravitropism (Tasaka). Transposon-taggad dwarf, and viviporous mutants of rice (Hirochika, Matsuoka). (2) Gene cloning from Arabidopsis mutants : HY5 (Okada), CAPRICE (Okada), CKI1 (Kakimoto), CUP-SHAPED COTYLEDON2 (Tasaka), SPIRAL1 (Hashimoto), and JAM (Machida). (3) Genetic analysis of MUTABLE gene of morning glory (Iida). (4) Biochemical and Molecular analysis of cell differentiation from leaf mesophyll cells to tracheary elements (Fukuda). (5) Screening of inflorescence-specific genes (Kohchi). (6) Generation of tagging system in Arabidopsis (Machida) or in rice (Hirochika). The results were published in international scientific journals and reported in conferences.

本项目旨在阐明利用拟南芥、水稻、牵牛花、百日菊和烟草等高等植物控制植物器官发生的遗传程序。(1)突变体的分离：影响花器官发生(Okada、Goto)、分生组织形成(Mchida、Tasaka)、叶发育(Mchida)、根生长(Okada)、根毛发育(Okada)、细胞分裂素非依赖性生长(Kakimoto)、温度敏感型新梢和/或根再生(Sugiyama)和新梢向重力性(Tasaka)的拟南芥突变体。转座子-Taggad矮秆和水稻的活孢子突变体(Hirochika，Matsuoka)。(2)拟南芥突变体的基因克隆：HY5(Okada)、Caprice(Okada)、CKI1(Kakimoto)、杯状COTYLEDON2(Tasaka)、SPIRAL1(Hashimoto)和Jam(Mchida)。(3)牵牛花突变基因(IIDA)的遗传分析。(4)叶片叶肉细胞向导管分子分化的生化和分子分析(福田)。(5)花序特异基因(Kohchi)的筛选。(6)拟南芥(Mchida)或水稻(Hirochika)标记系统的建立。研究结果发表在国际科学期刊上，并在会议上进行了报道。