Molecular Mechanisms Controlling Embryo and Meristem Formation

控制胚胎和分生组织形成的分子机制

• 批准号: 10182101
• 负责人: OKADA Kiyotaka
• 金额: $ 198.27万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10182101/
• 关键词: EMBRYO GENESIS; MERISTEM; REGULATORY GENE; PLANT ORGANOGENESIS; ARABIDOPSIS; ORYZA; ZINNIA; MUTANT

This project aimed to understand the genetic regulatory systems which contribute to make up multi-cellular body of plants, especially in the embryo development and in the meristem formation and maintenance. The major results are as follows.1. Okada group : Analysis of a root meristem-defective mutant, hlr, showed that it has a mutation in a subunit of proteosome complex. This is a novel observation showing that programmed protein degradation is required for maintaining structure and function of the root meristem. This group also examined FTR gene controlling polarized cell elongation, CPC gene controlling epidermal cell specification, KOM gene controlling male gametophyte, and DAD1 gene controlling jasmonic acid synthesis and anther opening. 2. Araki group studied FAS1 and 2 genes responding to the formation of shoot apical meristem (SAM). The genes encode subunits of chromatin assembly factor (CAF). The group also analyzed a key flowering gene, FT, and identified new genes suppressing … More or enhancing the effect of overexpressing FT. 3. Kakimoto group identified isopentenyl transferases are the key enzyme in the cytokinin biosynthesis. The group also studied cytokinin receptors and their downstream signaling pathways. 4. Shinozaki group : The group constructed a large number of transposon-tagged Arabidopsis lines, and studied a set of mutants showing male sterile, embryo lethal, leaf malmorphology. 5. Sugiyama group studied a set of mutnats showing temperature-dependent defects in the shoot and root regeneration process from callus. They cloned one of the genes, SRD2, and shown the gene is related to the transcriptional activation of snRNAs. 6. Tasaka group examined gene regulatory network controlling SAM formation in embryogenesis. They studied CUC1 and 2 genes and shown they regulate other SAM genes such as STM and KNOX. 7. Fukuda group : By using synchronized cell culture system of Xinnia and the tip-procedure, a large number of genes were identified which are required for vascular cell formation from leaf cells. Genes required for cell autolysis were also cloned. 8. Matsuoka group studied a variety of rice embryo pattern mutants showing many shoots, no shoots, aberrant organ form, and abberant orientation of meristem. The studies above were reported in journals or at international or national symposiums. Less

该项目旨在了解植物多细胞体的遗传调控系统，特别是在胚胎发育和分生组织的形成和维持中。主要研究结果如下：1. Okada组：对根分生组织缺陷突变体hlr的分析表明，它在蛋白体复合物的一个亚基上发生了突变。这是一个新的观察表明，程序化的蛋白质降解是维持根分生组织的结构和功能所必需的。本研究组还检测了控制极化细胞伸长的FTR基因、控制表皮细胞特化的CPC基因、控制雄配子体的KOM基因和控制茉莉酸合成和花药开放的DAD 1基因。2.荒木研究组研究了响应茎尖分生组织（SAM）形成的FAS 1和2基因。这些基因编码染色质组装因子（CAF）的亚基。该小组还分析了一个关键的开花基因FT，并确定了新的抑制基因， ...更多信息 或增强过表达FT的效果。3. Kakimoto等人发现异戊烯基转移酶是细胞分裂素生物合成的关键酶。该小组还研究了细胞分裂素受体及其下游信号通路。4.筱崎组：该小组构建了大量转座子标记的拟南芥品系，并研究了一组显示雄性不育、胚致死、叶片畸形的突变体。5. Sugiyama小组研究了一组在愈伤组织的芽和根再生过程中表现出温度依赖性缺陷的突变体。他们克隆了其中一个基因SRD 2，并表明该基因与snRNAs的转录激活有关。6. Tasaka小组研究了在胚胎发生中控制SAM形成的基因调控网络。他们研究了CUC 1和CUC 2基因，发现它们调节其他SAM基因，如STM和KNOX。7.福田集团：利用Xinnia的同步化细胞培养系统和tip-procedure，从叶片细胞中鉴定了大量维管细胞形成所需的基因。细胞自溶所需的基因也被克隆。8. Matsuoka研究组研究了一系列水稻胚型突变体，这些突变体表现为多芽、无芽、器官形态异常和分生组织方向异常。上述研究已在期刊或国际或国家研讨会上报告。少

项目成果

岡田清孝 他: "モデル植物ラボマニュアル"Springer-Verlag Tokyo. 275 (2000)

Kiyotaka Okada 等人：“模型植物实验室手册”Springer-Verlag 东京 275 (2000)。

S. Sawa: "Molecular Characterization of a meristem and organ identity gene of Arabidopsis, FILAMENTOUS FLOWER, encoding a zinc-finger and a HMG related domains"Gene & Development. 13. 1079-1088 (1999)

S. Sawa：“拟南芥、丝状花的分生组织和器官识别基因的分子特征，编码锌指和 HMG 相关结构域”基因

Tanaka-Ueguchi,M.: "Over-expression of a tobacco homeobox gene, NTH15, decreases the expression of a gibberellin biosynthetic gene encoding GA20-oxidase." Plant J.15. 391-400 (1998)

Tanaka-Ueguchi,M.：“烟草同源盒基因 NTH15 的过度表达会降低编码 GA20 氧化酶的赤霉素生物合成基因的表达。”

K.Koizumi: "A series of novel mutants of Arabidopsis thaliana that are defective in the formation of continuous vascular network"Development. 127. 3197-3204 (2000)

K.Koizumi：“一系列在形成连续维管网络方面存在缺陷的拟南芥新突变体”的开发。

S. Sawa: "FILAMENTOUS FLOWER controls formation and development of inflorescence and floral meristem of Arabidopsis thaliana"Plant Cell. 11. 69-86 (1999)

S. Sawa：“丝状花控制拟南芥花序和花分生组织的形成和发育”植物细胞。