Molecular Mechanism of Intercellular Signaling in Plant Organogenesis

植物器官发生中细胞间信号传导的分子机制

• 批准号: 08408031
• 负责人: OKADA Kiyotaka
• 金额: $ 19.14万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08408031/
• 关键词: PLANT ORGANOGENESIS; CELL DIFFERENTIATION; INTERCELLULAR SIGNALING; ARABIDOPSIS; MUTANT ANALYSIS; ROOT HAIR FORMATION; TRANSCRIPTION FACTOR; FLOWER DEVELOPMENT; 表裏の軸決定; 接触刺激; プラズモデデスマ-タ; トランスジェニック植物; mybドメイン; プラズモデスマ-タ

This project aimed to clarify intercellular signaling mechanism in plant organogenesis using several mutants of Arabidopsis. Major results obtained in the 3-year project are : (1) We identified and cloned a positive regulator, CPC, promoting differentiation of the root hair cells. CPC encodes a small myb protein, and found to be expressed in non-hair cells. It is strongly suggested that CPC protein moves to the neighboring hair cells. (2) HY5 gene was shown to have a role in photomorphogenesis, cell elongation, cell division, and chloroplast development. We cloned HY5 and found it encodes a bZIP protein, and associates with other photomorphogenesis regulatory proteins COP1 and DET1. (3) We cloned a flower gene, FIL, and found it encodes a protein with a zinc-finger and a HMG-related domains. FIL was expressed at the abaxial side tissues of leaves and of floral organs. Transgenic plants showed FIL has a role in determination of the abaxial side of lateral organs. (4) We identified a mutant with aberrant structure in meristems, L12, has two mutations. One gene, L12a, was found to encode a HSP90-related protein.

本项目旨在利用拟南芥的几个突变体来阐明植物器官发生中的细胞间信号转导机制。经过3年的研究，取得的主要成果有：(1)鉴定并克隆了一种促进根毛细胞分化的正性调控因子CPC。CPC编码一种小的MYB蛋白，并在非毛细胞中表达。这强烈地表明CPC蛋白向邻近的毛细胞移动。(2)HY5基因与光形态建成、细胞伸长、细胞分裂和叶绿体发育有关。我们克隆了HY5，发现它编码一个bZIP蛋白，并与其他光形态建成调控蛋白COP1和DET1结合。(3)克隆了花卉基因FIL，发现其编码一个含锌指结构域和HMG相关结构域的蛋白。FIL在叶片和花器官的远轴边部组织中表达。转基因植株表明FIL在决定侧器官的远轴方向上具有一定的作用。(4)我们发现了一个分生组织结构异常的突变体L12，它有两个突变。一个名为L12a的基因被发现编码一种HSP90相关蛋白。

项目成果

S. Sawa: "FILAMENTOUS FLOWER controls formation and development of inflorescence and floral meristem of Arabidopsis thaliana"Plant Cell. 11. 69-86 (1999)

S. Sawa：“丝状花控制拟南芥花序和花分生组织的形成和发育”植物细胞。

澤進一郎: "生殖細胞の発生と性分化" 共立出版, 622 (1997)

Shinichiro Sawa：“生殖细胞发育和性别分化” Kyoritsu Shuppan，622（1997）

岡田清孝: "植物の形態はどのように形作られるか 植物分子遺伝学の最近の成果" 生物の科学 遺伝. 別冊. 46-54 (1996)

Kiyotaka Okada：“植物形态是如何形成的？植物分子遗传学的最新成果”《生物科学遗传学》单独卷。

K.Okada, T.Wada, T.Oyama, M.Ohta, T.Tatsibana, K.Gohda, S.Ishiguro: "Regulatory systems of root patterning" J.Plant Res.111. 315-322 (1998)

K.Okada、T.Wada、T.Oyama、M.Ohta、T.Tatsibana、K.Gohda、S.Ishiguro：“根系图案的调控系统”J.Plant Res.111。

A.Tanaka: "Chlorophyll a oxygenase(CAO)is involved in chlorophyll b formation from chlorophyll a." Proc.Nalt.Acad,Sci.95. 12719-12723 (1998)

A.Tanaka：“叶绿素 a 加氧酶 (CAO) 参与叶绿素 a 形成叶绿素 b。”