Molecular Mechanism of Intercellular Signaling in Plant Organogenesis

植物器官发生中细胞间信号传导的分子机制

• 批准号: 08408031
• 负责人: OKADA Kiyotaka
• 金额: $ 19.14万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08408031/
• 关键词: PLANT ORGANOGENESIS; CELL DIFFERENTIATION; INTERCELLULAR SIGNALING; ARABIDOPSIS; MUTANT ANALYSIS; ROOT HAIR FORMATION; TRANSCRIPTION FACTOR; FLOWER DEVELOPMENT; 表裏の軸決定; 接触刺激; プラズモデデスマ-タ; トランスジェニック植物; mybドメイン; プラズモデスマ-タ

This project aimed to clarify intercellular signaling mechanism in plant organogenesis using several mutants of Arabidopsis. Major results obtained in the 3-year project are : (1) We identified and cloned a positive regulator, CPC, promoting differentiation of the root hair cells. CPC encodes a small myb protein, and found to be expressed in non-hair cells. It is strongly suggested that CPC protein moves to the neighboring hair cells. (2) HY5 gene was shown to have a role in photomorphogenesis, cell elongation, cell division, and chloroplast development. We cloned HY5 and found it encodes a bZIP protein, and associates with other photomorphogenesis regulatory proteins COP1 and DET1. (3) We cloned a flower gene, FIL, and found it encodes a protein with a zinc-finger and a HMG-related domains. FIL was expressed at the abaxial side tissues of leaves and of floral organs. Transgenic plants showed FIL has a role in determination of the abaxial side of lateral organs. (4) We identified a mutant with aberrant structure in meristems, L12, has two mutations. One gene, L12a, was found to encode a HSP90-related protein.

该项目旨在使用拟南芥的几个突变体阐明植物器官发生的细胞间信号传导机制。在3年项目中获得的主要结果是：（1）我们确定并克隆了一个阳性调节剂CPC，促进了根毛细胞的分化。 CPC编码一个小的MYB蛋白，并发现在非毛细胞中表达。强烈建议CPC蛋白移至相邻的毛细胞。 （2）显示HY5基因在光形态发生，细胞伸长，细胞分裂和叶绿体发育中起作用。我们克隆HY5并发现它编码了BZIP蛋白，并与其他光形态发生调节蛋白COP1和DET1相关联。 （3）我们克隆了一个花基因，并发现它编码了带有锌指和HMG相关域的蛋白质。 FIL在叶子和花器官的背面组织中表达。转基因植物显示FIL在确定侧侧器官的浅层侧的作用。 （4）我们确定了一个分生组织中具有异常结构的突变体，L12具有两个突变。发现一种基因L12a编码与Hsp90相关的蛋白质。

项目成果

岡田清孝: "植物の形態はどのように形作られるか 植物分子遺伝学の最近の成果" 生物の科学 遺伝. 別冊. 46-54 (1996)

Kiyotaka Okada：“植物形态是如何形成的？植物分子遗传学的最新成果”《生物科学遗传学》单独卷。

澤進一郎: "生殖細胞の発生と性分化" 共立出版, 622 (1997)

Shinichiro Sawa：“生殖细胞发育和性别分化” Kyoritsu Shuppan，622（1997）

A.Tanaka: "Chlorophyll a oxygenase(CAO)is involved in chlorophyll b formation from chlorophyll a." Proc.Nalt.Acad,Sci.95. 12719-12723 (1998)

A.Tanaka：“叶绿素 a 加氧酶 (CAO) 参与叶绿素 a 形成叶绿素 b。”

K.Okada, T.Wada, T.Oyama, M.Ohta, T.Tatsibana, K.Gohda, S.Ishiguro: "Regulatory systems of root patterning" J.Plant Res.111. 315-322 (1998)

K.Okada、T.Wada、T.Oyama、M.Ohta、T.Tatsibana、K.Gohda、S.Ishiguro：“根系图案的调控系统”J.Plant Res.111。

T.Ito: "A serine/threonine protein kinase gene isolated by in vivo binding procedure using Arabidopsis floral homeotic qene product,AGAMOUS." Plant & Cell Physiol.38. 248-258 (1997)

T.Ito：“使用拟南芥花同源异型基因产品 AGAMOUS 通过体内结合程序分离出丝氨酸/苏氨酸蛋白激酶基因。”