Development and architecture of the nervous system in Cephalocarida and Mystacocarida - Testing the monophyly of Crustacea

头蚴纲和须蚴纲神经系统的发育和结构 - 测试甲壳纲的单系性

• 批准号: 51421214
• 负责人: Professor Dr. Stefan Richter
• 金额: --
• 依托单位: Lehrstuhl für Allgemeine und Spezielle Zoologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/51421214?language=en
• 关键词: Development; architecture; nervous; system; Cephalocarida

The project continues to investigate the architecture and development of the nervous system in two crustacean taxa, Cephalocarida and Mystacocarida, using various kinds of immunohistochemical techniques. It has been suggested that both taxa are “primitive” crustaceans (i.e. characterized by a high number of plesiomorphic characters), and that Mystacocarida is even the sister group of all other Mandibulata (implying the paraphyly of Crustacea). The development of the nervous system in Hutchinsoniella macracantha (Cephalocarida), in Derocheilocaris remanei and in D. typica (Mystacocarida) will be studied using antibody staining against acetylated and tyrosinated α-tubulin and against synapsin. In addition, single serotonin, FMRFamide and histamine immunoreactive neurons will be characterized in order to reveal homologous cell patterns. Immunohistochemical techniques of this nature are now well established in various arthropods and potential sistergroups and allow maximum comparability between taxa. In addition, the gene expression pattern of even-skipped will be studied in combination with immunohistochemical techniques in order to identify single pioneer neurons which have played an important role in the debate on hexapod-crustacean relationships. In all these respects this study of cephalocarids and mystacocarids will fill important gaps in our understanding of deep arthropod and ultimately deep metazoan phylogeny.

该项目继续利用各种免疫组织化学技术，研究两个甲壳类分类群-囊虾目和囊虾目-的神经系统的结构和发育。有人认为这两个分类群都是“原始”的甲壳纲动物（即具有大量的近形特征），并且Mystacocarida甚至是所有其他Mandibulata的姐妹群（暗示甲壳纲的并系）。本文报道了大棘哈氏鱼（Hutchinsoniellamacracantha）、雷氏赤螯蟹（Derocheilocarisremanei）和大棘赤螯蟹（Derocheilocarisremanei）神经系统的发育。将使用针对乙酰化和酪氨酸化α-微管蛋白以及针对突触蛋白的抗体染色来研究typica（Mystacocarida）。此外，将表征单个5-羟色胺、FMRFamide和组胺免疫反应性神经元，以揭示同源细胞模式。这种性质的免疫组化技术，现在已经很好地建立在各种节肢动物和潜在的姐妹群，并允许最大的类群之间的可比性。此外，偶跳过的基因表达模式将结合免疫组织化学技术进行研究，以确定单一的先驱神经元，在辩论中发挥了重要作用的六足甲壳类动物的关系。在所有这些方面，这项研究的cephalocarids和mystacocarids将填补重要的空白，我们的理解深节肢动物，并最终深后生动物的生殖。