Enhancing Selectivity and Detection in Miniaturised Analytical Separation Systems

增强小型分析分离系统的选择性和检测

• 批准号: DP0452763
• 负责人: Prof Philip Marriott
• 金额: $ 15.26万
• 依托单位: RMIT University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0452763
• 关键词: Enhancing; Selectivity; Detection; Miniaturised; Analytical

Miniaturisation-compatible stationary phases and detection techniques will be developed for microseparation techniques of capillary electrochromatography and chip-based separation systems, and their analytical applications will be investigated. Replaceable stationary phases (RSP) of chromatographic particles suspended in reversible gels will be created by delivering a liquid RSP to the column followed by in situ immobilisation. This approach offers flexibility in optimising a number of parameters of the column and of its use for sample pre-treatment and preconcentration. Pulsed potentiometric detection will be developed for the abovementioned microseparation techniques, combining the advantages of pulsed amperometric techniques with the more universally responding potentiometric detection.

将为毛细管电色谱和芯片分离系统的微分离技术开发小型化兼容的固定相和检测技术，并研究其分析应用。悬浮在可逆凝胶中的色谱颗粒的可替换固定相（RSP）将通过将液体RSP输送到柱上，然后进行原位固定来创建。这种方法在优化色谱柱的一些参数及其用于样品预处理和预浓缩方面提供了灵活性。针对上述微分离技术，将开发脉冲电位检测技术，将脉冲安培技术的优点与更普遍响应的电位检测技术相结合。