Multi-Omics Core C

多OMICS CORE C摘要 这篇PPG讨论的是一个重要的假设，即早期生活压力(ELS)会导致从头到尾重新编程 身体导致成年后患脑血管病的风险增加。ELS和ELS之间的机械联系 成年期的心血管疾病和复原力尚未确立。转录或肠道微生物组的表观遗传调控 微生物衍生的因子被假设为对ELS敏感，并可能被修饰以调节 恢复力。多组学核心(核心C)的主要目标和功能是提供最先进的处理 转录组、DNA甲基组、DNA染色质可及性、代谢体、 以及所有项目所需的微生物群。核心的目标是提供一致性和严谨性 多组学实验并提供创新技术，以满足项目目标。具体目标 核心C是：目标1转录组和表观基因组测定。表观基因组是一个重要的 环境与每个细胞中表达的基因(转录本)之间的联系，并受到调控 通过DNA甲基化和染色质结构(可获得性)。核心C将有助于转录本的确定， 以及表观基因组全球DNA甲基化和染色质可及性。目标2：微生物组和 代谢物分析。在这个PPG中，假设ELS导致肠道微生物多样性的变化， 导致循环中的短链脂肪酸(SCFA)或其他代谢物发生显著变化。核心C将 准备微生物组16S测序样品，用于鉴定和量化微生物多样性。《核心》 还将准备样品用于SCFA和非靶向代谢物的质谱分析。目标3： 评估新技术，优化，同时促进财政责任。在这个多元经济学的时代， 技术正在迅速发展。因此，为了向项目提供最新的技术来测试他们的 假设，核心C将评估新的技术平台。核心C将针对样本优化所有协议 为以上所列的多重组学方法做准备。通过将多组体样品制备集中在 核心C，我们将批量购买试剂盒和试剂，从而节省大量成本。核心C将直接交互 与所有项目一起，从核心B接收临床样本，并直接与核心A合作进行样本鉴定， 管理、数据存储和分析以及数据管理。核心A将完成所有生物信息学和 对核心C生成的数据进行生物统计分析。核心C使用的方法确保PPG是 利用最新技术确定ELS导致心血管疾病风险的机制 板凳到旁边进场。

MULTI-OMICS CORE C SUMMARY This PPG is addressing an important hypothesis that early life stress (ELS) leads to reprogramming throughout the body resulting in an increased risk of developing CVD in adulthood. The mechanistic links between ELS and CVD and resilience in adulthood is not established. Epigenetic regulation of transcription or the gut microbiome and microbial-derived factors are hypothesized to be sensitive to ELS and may be modifiable to mediate resiliency. The primary goal and function of the Multi-omics Core (Core C) is to provide start-of-the-art processing of samples for determination of transcriptomes, DNA methylomes, DNA chromatin accessibility, metabolomes, and microbiomes required by all Projects. The objective of the Core is to provide consistency and rigor in the multi-omics experiments and provide innovative technologies to meet the goals of the Projects. The specific aims of Core C are: Aim 1 Transcriptomic and epigenomic determination. The epigenome is an important connection between the environment and the genes expressed in each cell (transcriptomes) and it is regulated by DNA methylation and chromatin structure (accessibility). Core C will facilitate determination of transcriptomes, and epigenome global DNA methylation and chromatin accessibility for the Projects. Aim 2: Microbiome and metabolite profiling. In this PPG, it is hypothesized that ELS results in gut microbial diversity changes that result in significant alterations to circulating short chain fatty acids (SCFA) or other metabolites. Core C will prepare samples for microbiome 16S sequencing for identifying and quantifying microbial diversity. The Core will also prepare samples for SCFA and untargeted metabolite determination by mass spectrometry. Aim 3: Evaluating new technologies, optimization while promoting fiscal responsibility. In this era of multi-omics, technologies are rapidly evolving. Thus, to provide the Projects with the latest technologies to test their hypotheses, Core C will evaluate new technological platforms. Core C will optimize all protocols for sample preparation for the multi-omics approaches listed above. By centralizing the mutli-omic sample preparation in Core C, we will purchase kits and reagents in bulk leading to substantial cost savings. Core C will interact directly with all Projects, receive clinical samples from Core B, and work directly with Core A for sample identification, curating, data deposition and analyses, and data management. Core A will complete all bioinformatics and biostatistical analyses for data generated in Core C. The approaches used in Core C ensure that the PPG is using the latest technologies for determining mechanisms of ELS induced cardiovascular disease risk with a bench to beside approach.