Imaging of properties of coherent elastic light scattering from turbid biological media

用相干的弹性散射光成像来可视化体内的厚组织形态，或深埋在混浊介质中的细胞，仍然是一个主要的挑战。我们采用了另一种成像光散射特性的方法，将细胞组织视为空间变化的折射率连续体，对散射光进行编码。我们提出了一种基于相位延迟、散斑和角度分辨散射来间接推断组织状态和形态的新方法。我们将在将原位上皮组织和细胞的结构和功能与光散射相关联方面开辟新的天地，例如检测埋在厚厚的混浊介质中的细胞的有丝分裂和凋亡，以及体内的癌症和癌前病变。

Imaging with coherent, elastically-scattered light for visualisation of thick-tissue morphology in vivo, or of cells buried deep in a turbid medium, remains a major challenge. We adopt an alternative approach of imaging of properties of light scattering based on regarding cellular tissue as a spatially-varying refractive-index continuum which encodes scattered light. We propose new methodologies to infer tissue state and morphology indirectly based on phase delay, speckle, and angle-resolved scattering. We will break new ground in correlating the structure and function of in situ epithelial tissue and cells to light scattering enabling, e.g., the detection of mitosis and apoptosis in cells buried in thick, turbid media, and of cancers and precancers in vivo.