Mass Cytometry: A breakthrough in multidimensional systems biology

质谱细胞术：多维系统生物学的突破

• 批准号: LE150100066
• 负责人: Prof David Sampson
• 金额: $ 29.34万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE150100066
• 关键词: Mass; Cytometry; breakthrough; multidimensional; systems

Mass cytometry - a breakthrough in multidimensional systems biology: Mass Cytometry by Time of Flight marries the resolution, specificity and sensitivity of atomic stable isotope mass spectrometry to the high-throughput, single-cell analytical advantages of flow cytometry. Using molecular probes conjugated with stable isotope tags, a large increase is possible in the number of simultaneous quantitative measurements in complex samples. These parameters, denoting cell type, function and signalling status, will make possible future advances in the understanding of the diversity of cell phenotype and function with a systems biology approach.

质谱流式细胞术-多维系统生物学的突破：飞行时间质谱流式细胞术将原子稳定同位素质谱的分辨率、特异性和灵敏度与流式细胞术的高通量、单细胞分析优势结合在一起。使用与稳定同位素标签缀合的分子探针，在复杂样品中的同时定量测量的数量有可能大幅增加。这些参数，表示细胞的类型，功能和信号状态，将使未来的进步，在理解细胞表型和功能的多样性与系统生物学方法。