4-Laser Cell Sorter

4 激光细胞分选仪

• 批准号: 517957028
• 金额: --
• 依托单位: Christian-Albrechts-Universität zu Kiel
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/517957028?language=en
• 关键词: Laser; Cell; Sorter

The department of Biology at Kiel University has a strong focus on experimental evolution, manipulation of the microbiome and the assessment of host cellular responses, the isolation and differentiation of bacteria, and single cell transcriptomics. Larger funding initiatives and several individual projects of a diversity of working groups aim to unravel host-microbe interactions and evolution. In the Biology Center of Kiel University, we currently lack an instrument that goes beyond the quantification of bacteria and eukaryotic cells over flow cytometry, and that would permit sorting of both prokaryotic as well as eukaryotic cells. The purchase of a Fluorescence Activated Cell Sorter would significantly advance our experimental capabilities at Kiel University and bring us on a new level in terms of manipulation of organismic interactions and cellular responses. The usage of the FACS would facilitate to a) understand and follow single or multiple bacterial strains and their interaction in the microbiome upon adding fluorescently labelled bacteria strains and viruses, b) the quantification of competing bacterial strains in evolution experiments and their re-isolation upon sorting of the single strains, that would permit further cultivation or e.g. infection experiments on the host to assess shifting virulence upon experimental evolution, c) quantify and sort host cells to start cell cultures or to assess their development or gene expression using e.g. single cell transcriptomics, and d) assess the expression of cellular signals and components and e) analyse cellular replication and cell cycle.

基尔大学的生物学系非常注重实验进化，微生物组的操纵和宿主细胞反应的评估，细菌的分离和分化以及单细胞转录组学。更大的资助计划和各种工作组的几个单独项目旨在揭示宿主-微生物的相互作用和进化。在基尔大学的生物中心，我们目前缺乏一种仪器，该仪器超出了流式细胞术对细菌和真核细胞的定量，并且允许对原核细胞和真核细胞进行分选。购买荧光激活细胞分选仪将大大提高我们在基尔大学的实验能力，并使我们在操纵器官相互作用和细胞反应方面达到一个新的水平。FACS的使用将有助于a）理解和跟踪单个或多个细菌菌株以及它们在添加荧光标记的细菌菌株和病毒后在微生物组中的相互作用，B）在进化实验中竞争细菌菌株的定量以及在分选单个菌株后它们的重新分离，这将允许对宿主进行进一步的培养或例如感染实验以评估实验进化后毒力的变化，c）定量和分选宿主细胞以开始细胞培养或使用例如单细胞转录组学评估其发育或基因表达，和d）评估细胞信号和组分的表达和e）分析细胞复制和细胞周期。