4-Laser Cell Sorter

基尔大学生物系的重点是实验进化，微生物组的操作和宿主细胞反应的评估，细菌的分离和分化，以及单细胞转录组学。更大的资助计划和多个工作组的几个单独项目旨在揭示宿主-微生物的相互作用和进化。在基尔大学的生物学中心，我们目前缺乏一种超越流式细胞术的细菌和真核细胞定量的仪器，它将允许对原核细胞和真核细胞进行分选。购买荧光活化细胞分选器将大大提高我们在基尔大学的实验能力，并使我们在操纵生物体相互作用和细胞反应方面达到一个新的水平。FACS的使用将有助于a)了解和跟踪单个或多个细菌菌株及其在添加荧光标记的细菌菌株和病毒后在微生物组中的相互作用，b)在进化实验中对竞争细菌菌株进行量化，并在对单个菌株进行分类后对其进行重新分离，这将允许进一步培养或在宿主上进行感染实验，以评估实验进化过程中毒力的变化。C)定量和分选宿主细胞以开始细胞培养或使用单细胞转录组学评估其发育或基因表达；d)评估细胞信号和成分的表达；e)分析细胞复制和细胞周期。

The department of Biology at Kiel University has a strong focus on experimental evolution, manipulation of the microbiome and the assessment of host cellular responses, the isolation and differentiation of bacteria, and single cell transcriptomics. Larger funding initiatives and several individual projects of a diversity of working groups aim to unravel host-microbe interactions and evolution. In the Biology Center of Kiel University, we currently lack an instrument that goes beyond the quantification of bacteria and eukaryotic cells over flow cytometry, and that would permit sorting of both prokaryotic as well as eukaryotic cells. The purchase of a Fluorescence Activated Cell Sorter would significantly advance our experimental capabilities at Kiel University and bring us on a new level in terms of manipulation of organismic interactions and cellular responses. The usage of the FACS would facilitate to a) understand and follow single or multiple bacterial strains and their interaction in the microbiome upon adding fluorescently labelled bacteria strains and viruses, b) the quantification of competing bacterial strains in evolution experiments and their re-isolation upon sorting of the single strains, that would permit further cultivation or e.g. infection experiments on the host to assess shifting virulence upon experimental evolution, c) quantify and sort host cells to start cell cultures or to assess their development or gene expression using e.g. single cell transcriptomics, and d) assess the expression of cellular signals and components and e) analyse cellular replication and cell cycle.