4-Laser Cell Sorter

4 激光细胞分选仪

• 批准号: 541916397
• 金额: --
• 依托单位: Rheinische Friedrich-Wilhelms-Universität Bonn
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/541916397?language=en
• 关键词: Laser; Cell; Sorter

Functional nucleic acids in the form of aptamers are gaining increasing importance in research, diagnostics, and therapy. In the Mayer research group, interdisciplinary projects focus on developing DNA and RNA aptamers that selectively bind target molecules on and within cells. These aptamers are used, for instance, for optogenetic control of cellular RNA, detection of metabolites within the cell, or targeted transport of other molecules to the cell. Conventional in vitro selection methods (SELEX) are based on the binding of aptamers to target molecules but do not allow direct selection for the desired function of aptamers on or within cells. With the requested cell sorter, novel selection methods are being established that enable the sorting of functional aptamers to target structures, making the process more streamlined and expedited. Furthermore, the properties of existing aptamers are re-analyzed and optimized within the relevant cellular environment using the cell sorter. Sorting of functional aptamer candidates is based on one or more fluorescence signals. Depending on their subsequent use, the samples are collected in reaction tubes, microplate wells, or onto slides.

适体形式的功能性核酸在研究、诊断和治疗中的重要性日益增加。在Mayer研究小组中，跨学科项目专注于开发选择性结合细胞上和细胞内靶分子的DNA和RNA适体。例如，这些适体用于细胞RNA的光遗传学控制、细胞内代谢物的检测或其他分子向细胞的靶向转运。常规的体外选择方法（SELEX）基于适体与靶分子的结合，但不允许直接选择细胞上或细胞内适体的所需功能。利用所要求的细胞分选仪，正在建立新的选择方法，使功能性适体能够分选到靶结构，使该过程更加简化和加快。此外，使用细胞分选仪在相关细胞环境中重新分析和优化现有适体的特性。功能性适体候选物的分选基于一个或多个荧光信号。根据其后续用途，将样品收集在反应管、微孔板威尔斯孔中或载玻片上。